Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy

Yu Jin Jung; Yu Shin Park; Ki Jun Yoon; Young Yun Kong; Joon Won Park; Hong Gil Nam

doi:10.1093/nar/gkn965

Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy

Yu Jin Jung, Yu Shin Park, Ki Jun Yoon, Young Yun Kong, Joon Won Park, Hong Gil Nam

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Detection of the cellular and tissue distributions of RNA species is critical in our understanding of the regulatory mechanisms underlying cellular and tissue differentiation. Here, we show that an atomic force microscope tip modified with 27-acid dendron, a cone shaped molecule with 27 monomeric units forming its base, can be successfully used to map the spatial distribution of mouse Pax6 mRNA on sectioned tissues of the mouse embryonic neocortex. Scanning of the sectioned tissue with a 30-mer DNA probe attached to the apex of the dendron resulted in detection of the target mRNA on the tissue section, permitting mapping of the mRNA distribution at nanometer resolution. The unprecedented sensitivity and resolution of this process should be applicable to identification of molecular level distribution of various RNAs in a cell.

Original language	English (US)
Article number	e10
Journal	Nucleic acids research
Volume	37
Issue number	2
DOIs	https://doi.org/10.1093/nar/gkn965
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy",

abstract = "Detection of the cellular and tissue distributions of RNA species is critical in our understanding of the regulatory mechanisms underlying cellular and tissue differentiation. Here, we show that an atomic force microscope tip modified with 27-acid dendron, a cone shaped molecule with 27 monomeric units forming its base, can be successfully used to map the spatial distribution of mouse Pax6 mRNA on sectioned tissues of the mouse embryonic neocortex. Scanning of the sectioned tissue with a 30-mer DNA probe attached to the apex of the dendron resulted in detection of the target mRNA on the tissue section, permitting mapping of the mRNA distribution at nanometer resolution. The unprecedented sensitivity and resolution of this process should be applicable to identification of molecular level distribution of various RNAs in a cell.",

author = "Jung, {Yu Jin} and Park, {Yu Shin} and Yoon, {Ki Jun} and Kong, {Young Yun} and Park, {Joon Won} and Nam, {Hong Gil}",

note = "Funding Information: J.W.P. acknowledges financial support from KOSEF through Center for Integrated Molecular Systems, NCRC, and the Brain Korea 21 program. H.G.N. acknowledges financial support by the Korea Science and Engineering Foundation (KOSEF); NCRC grant funded by the Korea government (MEST) (R15-2004-033-05002-0). Funding for open access charges: NCRC.",

year = "2009",

doi = "10.1093/nar/gkn965",

language = "English (US)",

volume = "37",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

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T1 - Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy

AU - Jung, Yu Jin

AU - Park, Yu Shin

AU - Yoon, Ki Jun

AU - Kong, Young Yun

AU - Park, Joon Won

AU - Nam, Hong Gil

N1 - Funding Information: J.W.P. acknowledges financial support from KOSEF through Center for Integrated Molecular Systems, NCRC, and the Brain Korea 21 program. H.G.N. acknowledges financial support by the Korea Science and Engineering Foundation (KOSEF); NCRC grant funded by the Korea government (MEST) (R15-2004-033-05002-0). Funding for open access charges: NCRC.

PY - 2009

Y1 - 2009

N2 - Detection of the cellular and tissue distributions of RNA species is critical in our understanding of the regulatory mechanisms underlying cellular and tissue differentiation. Here, we show that an atomic force microscope tip modified with 27-acid dendron, a cone shaped molecule with 27 monomeric units forming its base, can be successfully used to map the spatial distribution of mouse Pax6 mRNA on sectioned tissues of the mouse embryonic neocortex. Scanning of the sectioned tissue with a 30-mer DNA probe attached to the apex of the dendron resulted in detection of the target mRNA on the tissue section, permitting mapping of the mRNA distribution at nanometer resolution. The unprecedented sensitivity and resolution of this process should be applicable to identification of molecular level distribution of various RNAs in a cell.

AB - Detection of the cellular and tissue distributions of RNA species is critical in our understanding of the regulatory mechanisms underlying cellular and tissue differentiation. Here, we show that an atomic force microscope tip modified with 27-acid dendron, a cone shaped molecule with 27 monomeric units forming its base, can be successfully used to map the spatial distribution of mouse Pax6 mRNA on sectioned tissues of the mouse embryonic neocortex. Scanning of the sectioned tissue with a 30-mer DNA probe attached to the apex of the dendron resulted in detection of the target mRNA on the tissue section, permitting mapping of the mRNA distribution at nanometer resolution. The unprecedented sensitivity and resolution of this process should be applicable to identification of molecular level distribution of various RNAs in a cell.

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Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy

Abstract

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