Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometry

Bernhard Spengler; Ying Pan; Robert J. Cotter; Lou‐Sing ‐S Kan

doi:10.1002/rcm.1290040402

Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometry

Bernhard Spengler, Ying Pan, Robert J. Cotter, Lou‐Sing ‐S Kan

Research output: Contribution to journal › Article › peer-review

105 Scopus citations

Abstract

A Wiley‐McLaren type time‐of‐flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix‐assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive‐ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed.

Original language	English (US)
Pages (from-to)	99-102
Number of pages	4
Journal	Rapid Communications in Mass Spectrometry
Volume	4
Issue number	4
DOIs	https://doi.org/10.1002/rcm.1290040402
State	Published - Apr 1990
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy
Organic Chemistry

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10.1002/rcm.1290040402

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title = "Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometry",

abstract = "A Wiley‐McLaren type time‐of‐flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix‐assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive‐ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed.",

author = "Bernhard Spengler and Ying Pan and Cotter, {Robert J.} and Kan, {Lou‐Sing ‐S}",

year = "1990",

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AU - Spengler, Bernhard

AU - Pan, Ying

AU - Cotter, Robert J.

AU - Kan, Lou‐Sing ‐S

PY - 1990/4

Y1 - 1990/4

N2 - A Wiley‐McLaren type time‐of‐flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix‐assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive‐ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed.

AB - A Wiley‐McLaren type time‐of‐flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix‐assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive‐ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed.

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ER -

Molecular weight determination of underivatized oligodeoxyribonucleotides by positive‐ion matrix‐assisted ultraviolet laser‐desorption mass spectrometry

Abstract

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