Molecular tracking of antigen-specific T cell clones in neurological immune-mediated disorders

Paolo A. Muraro, Klaus Peter Wandinger, Bibiana Bielekova, Bruno Gran, Adriana Marques, Ursula Utz, Henry F. McFarland, Steve Jacobson, Roland Martin

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

T cells recognizing self or microbial antigens may trigger or reactivate immune-mediated diseases. Monitoring the frequency of specific T cell clonotypes to assess a possible link with the course of disease has been a difficult task with currently available technology. Our goal was to track individual candidate pathogenic T cell clones, selected on the basis of previous extensive studies from patients with immune-mediated disorders of the CNS, including multiple sclerosis, HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/ TSP) and chronic Lyme neuroborreliosis. We developed and applied a highly specific and sensitive technique to track single CD4+ and CD8+ T cell clones through the detection and quantification of T cell receptor (TCR) α or β chain complementarity-determining region 3 transcripts by real-time reverse transcriptase (RT)-PCR. We examined the frequency of the candidate pathogenic T cell clones in the peripheral blood and CSF during the course of neurological disease. Using this approach, we detected variations of clonal frequencies that appeared to be related to clinical course, significant enrichment in the CSF, or both. By integrating clono-type tracking with direct visualization of antigen-specific staining, we showed that a single T cell clone contributed substantially to the overall recognition of the viral peptide/MHC complex in a patient with HAM/ TSP. T cell clonotype tracking is a powerful new technology enabling further elucidation of the dynamics of expansion of autoreactive or pathogen-specific T cells that mediate pathological or protective immune responses in neurological disorders.

Original languageEnglish (US)
Pages (from-to)20-31
Number of pages12
JournalBrain
Volume126
Issue number1
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Keywords

  • Autoimmunity
  • HAM/TSP
  • Immunotherapy
  • Lyme disease
  • Multiple sclerosis
  • T cell receptors

ASJC Scopus subject areas

  • General Neuroscience

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