Molecular testing for Chlamydia trachomatis and Neisseria gonorrhoeae

Chlamydia trachomatis and Neisseria gonorrhoeae are currently the two most prevalent pathogens in the United States. The costs of infections, to individuals and society, are significant. Unfortunately, these pathogens can be difficult to detect with conventional diagnostic methods. New molecular techniques have improved diagnosis and have made screening possible. This aim of this chapter is to provide practical information on molecular testing for C. trachomatis and N. gonorrhoeae that is useful to practitioners of molecular pathology. Introductory information on the clinical significance and conventional diagnostic tests is provided. Issues confronting molecular testing for these pathogens are discussed. Descriptions of each molecular platform are provided, including performance characteristics and other parameters of functionality.