Molecular identification of an IgE-dependent histamine-releasing factor

Susan M. MacDonald; Thorunn Rafnar; Jacqueline Langdon; Lawrence M. Lichtenstein

doi:10.1126/science.7542803

Molecular identification of an IgE-dependent histamine-releasing factor

Susan M. MacDonald, Thorunn Rafnar, Jacqueline Langdon, Lawrence M. Lichtenstein

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313 Scopus citations

Abstract

An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.

Original language	English (US)
Pages (from-to)	688-690
Number of pages	3
Journal	Science
Volume	269
Issue number	5224
DOIs	https://doi.org/10.1126/science.7542803
State	Published - 1995
Externally published	Yes

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title = "Molecular identification of an IgE-dependent histamine-releasing factor",

abstract = "An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.",

author = "MacDonald, {Susan M.} and Thorunn Rafnar and Jacqueline Langdon and Lichtenstein, {Lawrence M.}",

year = "1995",

doi = "10.1126/science.7542803",

language = "English (US)",

volume = "269",

pages = "688--690",

journal = "Science",

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T1 - Molecular identification of an IgE-dependent histamine-releasing factor

AU - MacDonald, Susan M.

AU - Rafnar, Thorunn

AU - Langdon, Jacqueline

AU - Lichtenstein, Lawrence M.

PY - 1995

Y1 - 1995

N2 - An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.

AB - An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.

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DO - 10.1126/science.7542803

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VL - 269

SP - 688

EP - 690

JO - Science

JF - Science

IS - 5224

ER -

Molecular identification of an IgE-dependent histamine-releasing factor

Abstract

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