TY - JOUR
T1 - Molecular epidemiology of O139 Vibrio cholerae
T2 - Mutation, lateral gene transfer, and founder flush
AU - Garg, Pallavi
AU - Aydanian, Antonia
AU - Smith, David
AU - Morris, J. Glenn
AU - Nair, G. Balakrish
AU - Stine, O. Colin
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Vibrio cholerae in O-group 139 was first isolated in 1992 and by 1993 had been found throughout the Indian subcontinent. This epidemic expansion probably resulted from a single source after a lateral gene transfer (LGT) event that changed the serotype of an epidemic V. cholerae O1 El Tor strain to O139. However, some studies found substantial genetic diversity, perhaps caused by multiple origins. To further explore the relatedness of O139 strains, we analyzed nine sequenced loci from 96 isolates from patients at the Infectious Diseases Hospital, Calcutta, from 1992 to 2000. We found 64 novel alleles distributed among 51 sequence types. LGT events produced three times the number of nucleotide changes compared to mutation. In contrast to the traditional concept of epidemic spread of a homogeneous clone, the establishment of variant alleles generated by LGT during the rapid expansion of a clonal bacterial population may be a paradigm in infections and epidemics.
AB - Vibrio cholerae in O-group 139 was first isolated in 1992 and by 1993 had been found throughout the Indian subcontinent. This epidemic expansion probably resulted from a single source after a lateral gene transfer (LGT) event that changed the serotype of an epidemic V. cholerae O1 El Tor strain to O139. However, some studies found substantial genetic diversity, perhaps caused by multiple origins. To further explore the relatedness of O139 strains, we analyzed nine sequenced loci from 96 isolates from patients at the Infectious Diseases Hospital, Calcutta, from 1992 to 2000. We found 64 novel alleles distributed among 51 sequence types. LGT events produced three times the number of nucleotide changes compared to mutation. In contrast to the traditional concept of epidemic spread of a homogeneous clone, the establishment of variant alleles generated by LGT during the rapid expansion of a clonal bacterial population may be a paradigm in infections and epidemics.
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U2 - 10.3201/eid0907.020760
DO - 10.3201/eid0907.020760
M3 - Article
C2 - 12890320
AN - SCOPUS:0037710467
SN - 1080-6040
VL - 9
SP - 810
EP - 814
JO - Emerging infectious diseases
JF - Emerging infectious diseases
IS - 7
ER -