Molecular epidemiology of HIV type 1 in Singapore and identification of novel CRF01-AE/B recombinant forms

Oon Tek Ng; Supriya Munshaw; Susanna L. Lamers; Kuan Kiat Chew; Li Lin; Andrew D. Redd; Jordyn Manucci; Thomas C. Quinn; Stuart C. Ray; Arlene Chua; Yee Sin Leo; Oliver Laeyendecker

doi:10.1089/aid.2010.0364

Molecular epidemiology of HIV type 1 in Singapore and identification of novel CRF01-AE/B recombinant forms

Oon Tek Ng, Supriya Munshaw, Susanna L. Lamers, Kuan Kiat Chew, Li Lin, Andrew D. Redd, Jordyn Manucci, Thomas C. Quinn, Stuart C. Ray, Arlene Chua, Yee Sin Leo, Oliver Laeyendecker

School of Medicine

Research output: Contribution to journal › Review article › peer-review

13 Scopus citations

Abstract

To investigate HIV-1 molecular epidemiology in Singapore, we sequenced portions of three regions of the HIV-1 genome (protease HXB2: 2163 to 2620, gp120 HXB2: 6904 to 7628, and gp41 HXB2: 7817 to 8264) from 212 plasma samples collected between February 2008 and August 2009. From these samples, 109 (51.4%) generated interpretable data in all regions. Sixty-one (56.0%) were identified as CRF01AE, 26 (23.9%) as subtype B and 14 (12.8%) as possible novel recombinant forms. The main novel recombinant pattern, detected in 13 sequences, had subtype B in protease and gp41 and CRF01-AE in gp120. There was intermixing of subtypes within transmission risk groups. However, 85% of subjects infected with the novel recombinant forms self-identified as men who have sex with men or bisexuals compared with only 41% of individuals infected with CRF01-AE and 62% infected with subtype B (p = 0.001).

Original language	English (US)
Pages (from-to)	1135-1137
Number of pages	3
Journal	AIDS research and human retroviruses
Volume	27
Issue number	10
DOIs	https://doi.org/10.1089/aid.2010.0364
State	Published - Oct 1 2011

ASJC Scopus subject areas

Immunology
Virology
Infectious Diseases

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title = "Molecular epidemiology of HIV type 1 in Singapore and identification of novel CRF01-AE/B recombinant forms",

abstract = "To investigate HIV-1 molecular epidemiology in Singapore, we sequenced portions of three regions of the HIV-1 genome (protease HXB2: 2163 to 2620, gp120 HXB2: 6904 to 7628, and gp41 HXB2: 7817 to 8264) from 212 plasma samples collected between February 2008 and August 2009. From these samples, 109 (51.4%) generated interpretable data in all regions. Sixty-one (56.0%) were identified as CRF01AE, 26 (23.9%) as subtype B and 14 (12.8%) as possible novel recombinant forms. The main novel recombinant pattern, detected in 13 sequences, had subtype B in protease and gp41 and CRF01-AE in gp120. There was intermixing of subtypes within transmission risk groups. However, 85% of subjects infected with the novel recombinant forms self-identified as men who have sex with men or bisexuals compared with only 41% of individuals infected with CRF01-AE and 62% infected with subtype B (p = 0.001).",

author = "Ng, {Oon Tek} and Supriya Munshaw and Lamers, {Susanna L.} and Chew, {Kuan Kiat} and Li Lin and Redd, {Andrew D.} and Jordyn Manucci and Quinn, {Thomas C.} and Ray, {Stuart C.} and Arlene Chua and Leo, {Yee Sin} and Oliver Laeyendecker",

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AU - Lin, Li

AU - Redd, Andrew D.

AU - Manucci, Jordyn

AU - Quinn, Thomas C.

AU - Ray, Stuart C.

AU - Chua, Arlene

AU - Leo, Yee Sin

AU - Laeyendecker, Oliver

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N2 - To investigate HIV-1 molecular epidemiology in Singapore, we sequenced portions of three regions of the HIV-1 genome (protease HXB2: 2163 to 2620, gp120 HXB2: 6904 to 7628, and gp41 HXB2: 7817 to 8264) from 212 plasma samples collected between February 2008 and August 2009. From these samples, 109 (51.4%) generated interpretable data in all regions. Sixty-one (56.0%) were identified as CRF01AE, 26 (23.9%) as subtype B and 14 (12.8%) as possible novel recombinant forms. The main novel recombinant pattern, detected in 13 sequences, had subtype B in protease and gp41 and CRF01-AE in gp120. There was intermixing of subtypes within transmission risk groups. However, 85% of subjects infected with the novel recombinant forms self-identified as men who have sex with men or bisexuals compared with only 41% of individuals infected with CRF01-AE and 62% infected with subtype B (p = 0.001).

AB - To investigate HIV-1 molecular epidemiology in Singapore, we sequenced portions of three regions of the HIV-1 genome (protease HXB2: 2163 to 2620, gp120 HXB2: 6904 to 7628, and gp41 HXB2: 7817 to 8264) from 212 plasma samples collected between February 2008 and August 2009. From these samples, 109 (51.4%) generated interpretable data in all regions. Sixty-one (56.0%) were identified as CRF01AE, 26 (23.9%) as subtype B and 14 (12.8%) as possible novel recombinant forms. The main novel recombinant pattern, detected in 13 sequences, had subtype B in protease and gp41 and CRF01-AE in gp120. There was intermixing of subtypes within transmission risk groups. However, 85% of subjects infected with the novel recombinant forms self-identified as men who have sex with men or bisexuals compared with only 41% of individuals infected with CRF01-AE and 62% infected with subtype B (p = 0.001).

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Molecular epidemiology of HIV type 1 in Singapore and identification of novel CRF01-AE/B recombinant forms

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