TY - JOUR
T1 - Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers
AU - Liu, Yu
AU - Wang, Jingxing
AU - Huang, Yi
AU - Yang, Tonghan
AU - Guo, Xiaoming
AU - Li, Julin
AU - Wen, Guoxin
AU - Yun, Zhongqiao
AU - Zeng, Peibin
AU - He, Miao
AU - Xu, Min
AU - Liu, Gui
AU - Ke, Ling
AU - Wright, David
AU - Liu, Jing
AU - Nelson, Kenrad
AU - Shan, Hua
N1 - Funding Information:
This work was supported by NHLBI Retroviral Epidemiology Donor Study-II [RFP-05-8067].
PY - 2012/9
Y1 - 2012/9
N2 - Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.
AB - Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.
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U2 - 10.1007/s00705-012-1331-x
DO - 10.1007/s00705-012-1331-x
M3 - Article
C2 - 22669316
AN - SCOPUS:84865748677
SN - 0304-8608
VL - 157
SP - 1699
EP - 1707
JO - Archives of Virology
JF - Archives of Virology
IS - 9
ER -