Molecular diagnostics for personal medicine using a nanopore

Utkur M. Mirsaidov, Deqiang Wang, Winston Timp, Gregory Timp

Research output: Contribution to journalReview articlepeer-review

Abstract

Semiconductor nanotechnology has created the ultimate analytical tool: a nanopore with single molecule sensitivity. This tool offers the intriguing possibility of high-hroughput, low cost sequencing of DNA with the absolute minimum of material and preprocessing. The exquisite single molecule sensitivity obviates the need for costly and error-prone procedures like polymerase chain reaction amplification. Instead, nanopore sequencing relies on the electric signal that develops when a DNA molecule translocates through a pore in a membrane. If each base pair has a characteristic electrical signature, then ostensibly a pore could be used to analyze the sequence by reporting all of the signatures in a single read without resorting to multiple DNA copies. The potential for a long read length combined with high translocation velocity should make resequencing inexpensive and allow for haplotyping and methylation profiling in a chromosome.

Original languageEnglish (US)
Pages (from-to)367-381
Number of pages15
JournalWiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
Volume2
Issue number4
DOIs
StatePublished - Jul 2010
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

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