TY - JOUR
T1 - Molecular detection and characterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines
AU - Liu, Xiaofang
AU - Yamamoto, Dai
AU - Saito, Mariko
AU - Imagawa, Toshifumi
AU - Ablola, Adrianne
AU - Tandoc, Amado O.
AU - Segubre-Mercado, Edelwisa
AU - Lupisan, Socorro P.
AU - Okamoto, Michiko
AU - Furuse, Yuki
AU - Saito, Mayuko
AU - Oshitani, Hitoshi
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Background: Human sapovirus (SaV) is a causative agent of acute gastroenteritis. Recently, SaV detection has been increasing worldwide due to the emerging SaV genotype I.2. However, SaV infection has not been reported in the Philippines. Objectives: To evaluate the prevalence and genetic diversity of SaV in hospitalized children aged less than 5 years with acute gastroenteritis. Study design: Stool samples were collected from children with acute gastroenteritis at three hospitals in the Philippines from June 2012 to August 2013. SaV was detected by reverse transcription real-time PCR, and the polymerase and capsid gene sequences were analyzed. Full genome sequencing and recombination analysis were performed on possible recombinant viruses. Results: SaV was detected in 7.0% of the tested stool samples (29/417). In 10 SaV-positive cases, other viruses were also detected, including rotavirus (n=6), norovirus (n=2), and human astrovirus (n=2). Four known SaV genotypes (GI.1 [7], GI.2 [2], GII.1 [12], and GV [2]) and one novel recombinant (n=3) were identified by polymerase and capsid gene sequence analysis. Full genome sequencing revealed that the 5[U+02B9] nontranslated region (NTR) and nonstructural protein region of the novel recombinant were closely related to the GII.1 Bristol/98/UK variant, whereas the structural protein region and 3[U+02B9] NTR were closely related to the GII.4 Kumamoto6/Mar2003/JPN variant. Discussion and conclusions: SaV was regularly detected in hospitalized children due to acute gastroenteritis during the study period. A novel recombinant, SaV GII.1/GII.4, was identified in three cases at two different study sites.
AB - Background: Human sapovirus (SaV) is a causative agent of acute gastroenteritis. Recently, SaV detection has been increasing worldwide due to the emerging SaV genotype I.2. However, SaV infection has not been reported in the Philippines. Objectives: To evaluate the prevalence and genetic diversity of SaV in hospitalized children aged less than 5 years with acute gastroenteritis. Study design: Stool samples were collected from children with acute gastroenteritis at three hospitals in the Philippines from June 2012 to August 2013. SaV was detected by reverse transcription real-time PCR, and the polymerase and capsid gene sequences were analyzed. Full genome sequencing and recombination analysis were performed on possible recombinant viruses. Results: SaV was detected in 7.0% of the tested stool samples (29/417). In 10 SaV-positive cases, other viruses were also detected, including rotavirus (n=6), norovirus (n=2), and human astrovirus (n=2). Four known SaV genotypes (GI.1 [7], GI.2 [2], GII.1 [12], and GV [2]) and one novel recombinant (n=3) were identified by polymerase and capsid gene sequence analysis. Full genome sequencing revealed that the 5[U+02B9] nontranslated region (NTR) and nonstructural protein region of the novel recombinant were closely related to the GII.1 Bristol/98/UK variant, whereas the structural protein region and 3[U+02B9] NTR were closely related to the GII.4 Kumamoto6/Mar2003/JPN variant. Discussion and conclusions: SaV was regularly detected in hospitalized children due to acute gastroenteritis during the study period. A novel recombinant, SaV GII.1/GII.4, was identified in three cases at two different study sites.
KW - Gastroenteritis
KW - Recombination
KW - Sapovirus
KW - The Philippines
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U2 - 10.1016/j.jcv.2015.05.001
DO - 10.1016/j.jcv.2015.05.001
M3 - Article
C2 - 26071343
AN - SCOPUS:84930520696
SN - 1386-6532
VL - 68
SP - 83
EP - 88
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
ER -