Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India

R. Kannangai, S. Ramalingam, K. J. Prakash, O. C. Abraham, R. George, R. C. Castillo, D. H. Schwartz, M. V. Jesudason, G. Sridharan

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.

Original languageEnglish (US)
Pages (from-to)987-989
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number6
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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