Molecular cloning of the murine branched chain α-ketoacid dehydrogenase E2 subunit: Presence of 3′ B1 repeat elements

Paul A. Costeas; Lois A. Tonelli; Jeffrey M. Chinsky

doi:10.1016/0167-4781(95)00212-x

Molecular cloning of the murine branched chain α-ketoacid dehydrogenase E2 subunit: Presence of 3′ B1 repeat elements

Paul A. Costeas, Lois A. Tonelli, Jeffrey M. Chinsky

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain α-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5′ sequence encoding the amino-terminal preprotein sequence and 3′ untranslated region are less well conserved. The 3′-noncoding region contains sequences highly homologous to the rodent BI repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3′-noncoding region of human E2 cDNA.

Original language	English (US)
Pages (from-to)	25-28
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1305
Issue number	1-2
DOIs	https://doi.org/10.1016/0167-4781(95)00212-x
State	Published - Feb 7 1996
Externally published	Yes

Keywords

B1 repeat
Branched-chain α-ketoacid dehydrogenase
Dihydrolipoyl transacylase
E2 subunit

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(95)00212-x

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title = "Molecular cloning of the murine branched chain α-ketoacid dehydrogenase E2 subunit: Presence of 3′ B1 repeat elements",

abstract = "The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain α-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5′ sequence encoding the amino-terminal preprotein sequence and 3′ untranslated region are less well conserved. The 3′-noncoding region contains sequences highly homologous to the rodent BI repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3′-noncoding region of human E2 cDNA.",

keywords = "B1 repeat, Branched-chain α-ketoacid dehydrogenase, Dihydrolipoyl transacylase, E2 subunit",

author = "Costeas, {Paul A.} and Tonelli, {Lois A.} and Chinsky, {Jeffrey M.}",

note = "Funding Information: We thank Yu Zhao and Robert Harris, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN for sharing their cloned rat BCKAD E2 cDNA sequence with us \[5\]. The murine skeletal muscle and liver AZAP cDNA libraries were generously supplied by the laboratory of C. Thomas Caskey, Baylor College of Medicine, Houston, TX. This work was supported in part by grants from the Life and Health Insurance Medical Research Fund and the Frank C. Bressler Research Fund of the University of Maryland School of Medicine.",

year = "1996",

month = feb,

day = "7",

doi = "10.1016/0167-4781(95)00212-x",

language = "English (US)",

volume = "1305",

pages = "25--28",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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T1 - Molecular cloning of the murine branched chain α-ketoacid dehydrogenase E2 subunit

T2 - Presence of 3′ B1 repeat elements

AU - Costeas, Paul A.

AU - Tonelli, Lois A.

AU - Chinsky, Jeffrey M.

N1 - Funding Information: We thank Yu Zhao and Robert Harris, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN for sharing their cloned rat BCKAD E2 cDNA sequence with us \[5\]. The murine skeletal muscle and liver AZAP cDNA libraries were generously supplied by the laboratory of C. Thomas Caskey, Baylor College of Medicine, Houston, TX. This work was supported in part by grants from the Life and Health Insurance Medical Research Fund and the Frank C. Bressler Research Fund of the University of Maryland School of Medicine.

PY - 1996/2/7

Y1 - 1996/2/7

N2 - The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain α-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5′ sequence encoding the amino-terminal preprotein sequence and 3′ untranslated region are less well conserved. The 3′-noncoding region contains sequences highly homologous to the rodent BI repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3′-noncoding region of human E2 cDNA.

AB - The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain α-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5′ sequence encoding the amino-terminal preprotein sequence and 3′ untranslated region are less well conserved. The 3′-noncoding region contains sequences highly homologous to the rodent BI repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3′-noncoding region of human E2 cDNA.

KW - B1 repeat

KW - Branched-chain α-ketoacid dehydrogenase

KW - Dihydrolipoyl transacylase

KW - E2 subunit

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ER -

Molecular cloning of the murine branched chain α-ketoacid dehydrogenase E2 subunit: Presence of 3′ B1 repeat elements

Abstract

Keywords

ASJC Scopus subject areas

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