Abstract
The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transacylase) of the branched-chain α-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted amino acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5′ sequence encoding the amino-terminal preprotein sequence and 3′ untranslated region are less well conserved. The 3′-noncoding region contains sequences highly homologous to the rodent BI repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3′-noncoding region of human E2 cDNA.
Original language | English (US) |
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Pages (from-to) | 25-28 |
Number of pages | 4 |
Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
Volume | 1305 |
Issue number | 1-2 |
DOIs | |
State | Published - Feb 7 1996 |
Externally published | Yes |
Keywords
- B1 repeat
- Branched-chain α-ketoacid dehydrogenase
- Dihydrolipoyl transacylase
- E2 subunit
ASJC Scopus subject areas
- Structural Biology
- Biophysics
- Biochemistry
- Genetics