Molecular cloning of human genes for serum amyloid A

George H. Sack

doi:10.1016/0378-1119(83)90143-9

Molecular cloning of human genes for serum amyloid A

George H. Sack

School of Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

Original language	English (US)
Pages (from-to)	19-24
Number of pages	6
Journal	Gene
Volume	21
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(83)90143-9
State	Published - 1983

Keywords

Recombinant DNA
acute phase response
amyloidosis
plaque hybridization

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(83)90143-9

Cite this

@article{63a652823f054863afab797ed9573554,

title = "Molecular cloning of human genes for serum amyloid A",

abstract = "Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.",

keywords = "Recombinant DNA, acute phase response, amyloidosis, plaque hybridization",

author = "Sack, {George H.}",

note = "Funding Information: The author is especially grateful to Dr. John Morrow for many helpful discussions, to Dr. Ro- bert Stearman for a gift of plasmid pRS48 and sharing of his data, to Dr. William Pearson for assistancew ith computer analysis and to Dr. Kirby Smith for space to perform phage isolations. The work has been supported by USPHS Grant NINCDS No. lPOlNSl6375-02 and by generous gifts from Mr. Daniel M. Kelly and Mr. and Mrs. William M. Griffin.",

year = "1983",

doi = "10.1016/0378-1119(83)90143-9",

language = "English (US)",

volume = "21",

pages = "19--24",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Molecular cloning of human genes for serum amyloid A

AU - Sack, George H.

N1 - Funding Information: The author is especially grateful to Dr. John Morrow for many helpful discussions, to Dr. Ro- bert Stearman for a gift of plasmid pRS48 and sharing of his data, to Dr. William Pearson for assistancew ith computer analysis and to Dr. Kirby Smith for space to perform phage isolations. The work has been supported by USPHS Grant NINCDS No. lPOlNSl6375-02 and by generous gifts from Mr. Daniel M. Kelly and Mr. and Mrs. William M. Griffin.

PY - 1983

Y1 - 1983

N2 - Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

AB - Three human DNA fragments hybridizing to a mouse cDNA plasmid for the acute phase protein amyloid A have been isolated from the human λ Charon 4A phage library. Two of these recombinants, GSAA1 (12.8 kb insert) and GSAA2 (15.9 kb insert), share an apparently identical internal region of 9.7 kb while the third, GSAA3 (15.95-kb insert) shows different restriction enzyme fragments. Hybridization studies localize the coding region to single HindIII fragments and suggest that all coding information is present in these recombinants; these fragments have been subcloned into pBR322 and mapped for further study.

KW - Recombinant DNA

KW - acute phase response

KW - amyloidosis

KW - plaque hybridization

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DO - 10.1016/0378-1119(83)90143-9

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AN - SCOPUS:0020548931

SN - 0378-1119

VL - 21

SP - 19

EP - 24

JO - Gene

JF - Gene

IS - 1-2

ER -

Molecular cloning of human genes for serum amyloid A

Abstract

Keywords

ASJC Scopus subject areas

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