Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

Andrea Rasola; Luis J.V. Galietta; Virginia Barone; Giovanni Romeo; Serena Bagnasco

doi:10.1016/0014-5793(95)01052-G

Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

Andrea Rasola, Luis J.V. Galietta, Virginia Barone, Giovanni Romeo, Serena Bagnasco

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a K_m = 20 μM for GABA transport and a coupling to Na⁺ and Cl^- with a stoichiometry 3 Na⁺:2 Cl^-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

Original language	English (US)
Pages (from-to)	229-233
Number of pages	5
Journal	FEBS Letters
Volume	373
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01052-G
State	Published - Oct 16 1995
Externally published	Yes

Keywords

Betaine
Cell volume
Chromosomal localization
GABA
Kidney
Transporter

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)01052-G

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title = "Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney",

abstract = "The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.",

keywords = "Betaine, Cell volume, Chromosomal localization, GABA, Kidney, Transporter",

author = "Andrea Rasola and Galietta, {Luis J.V.} and Virginia Barone and Giovanni Romeo and Serena Bagnasco",

note = "Funding Information: Acknowledgements: This work was supported in part from grants from AIRC (Associazione Italiana per la Ricerca sul Cancro) to L.J.V.G. We would like to thank Dr. Olga Zegarra-Moran for help in data analysis and for reading the manuscript, and Giuseppe Santamaria for technical assistance.",

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doi = "10.1016/0014-5793(95)01052-G",

language = "English (US)",

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T1 - Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

AU - Rasola, Andrea

AU - Galietta, Luis J.V.

AU - Barone, Virginia

AU - Romeo, Giovanni

AU - Bagnasco, Serena

N1 - Funding Information: Acknowledgements: This work was supported in part from grants from AIRC (Associazione Italiana per la Ricerca sul Cancro) to L.J.V.G. We would like to thank Dr. Olga Zegarra-Moran for help in data analysis and for reading the manuscript, and Giuseppe Santamaria for technical assistance.

PY - 1995/10/16

Y1 - 1995/10/16

N2 - The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

AB - The human homologue of the canine GABA/betaine transporter (BGT-1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a Km = 20 μM for GABA transport and a coupling to Na+ and Cl- with a stoichiometry 3 Na+:2 Cl-:1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.

KW - Betaine

KW - Cell volume

KW - Chromosomal localization

KW - GABA

KW - Kidney

KW - Transporter

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EP - 233

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney

Abstract

Keywords

ASJC Scopus subject areas

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