Molecular cloning and expression of the cDNA for the hamster α1-adrenergic receptor

S. Cotecchia, D. A. Schwinn, R. R. Randall, R. J. Lefkowitz, M. G. Caron, B. K. Kobilka

Research output: Contribution to journalArticlepeer-review

476 Scopus citations


The cDNA for the Syrian hamster α1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the α1-adrenergic receptor that couples to inositol phospholipid metabolism.

Original languageEnglish (US)
Pages (from-to)7159-7163
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number19
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • General


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