Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana

Horácio M. Frydman; Esteban Osorio Cadavid; Jonny Yokosawa; Flavio Henrique Silva; Lucy Deise Navarro-Cattapan; Roberto Vicente Santelli; Marcelo Jacobs-Lorena; Monika Graessmann; Adolf Graessmann; Ann Jacob Stocker; F. J.S. Lara

doi:10.1006/jmbi.1993.1556

Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana

Horácio M. Frydman, Esteban Osorio Cadavid, Jonny Yokosawa, Flavio Henrique Silva, Lucy Deise Navarro-Cattapan, Roberto Vicente Santelli, Marcelo Jacobs-Lorena, Monika Graessmann, Adolf Graessmann, Ann Jacob Stocker, F. J.S. Lara

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promoter region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.

Original language	English (US)
Pages (from-to)	799-803
Number of pages	5
Journal	Journal of molecular biology
Volume	233
Issue number	4
DOIs	https://doi.org/10.1006/jmbi.1993.1556
State	Published - Dec 20 1993
Externally published	Yes

Keywords

C-8 cDNA
C-8 mrNA
DNA puff
Rhynchosciara
Salivary glands

ASJC Scopus subject areas

Molecular Biology
Biophysics
Structural Biology

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10.1006/jmbi.1993.1556

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title = "Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana",

abstract = "We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promoter region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.",

keywords = "C-8 cDNA, C-8 mrNA, DNA puff, Rhynchosciara, Salivary glands",

author = "Frydman, {Hor{\'a}cio M.} and Cadavid, {Esteban Osorio} and Jonny Yokosawa and Silva, {Flavio Henrique} and Navarro-Cattapan, {Lucy Deise} and Santelli, {Roberto Vicente} and Marcelo Jacobs-Lorena and Monika Graessmann and Adolf Graessmann and Stocker, {Ann Jacob} and Lara, {F. J.S.}",

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language = "English (US)",

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AU - Frydman, Horácio M.

AU - Cadavid, Esteban Osorio

AU - Yokosawa, Jonny

AU - Silva, Flavio Henrique

AU - Navarro-Cattapan, Lucy Deise

AU - Santelli, Roberto Vicente

AU - Jacobs-Lorena, Marcelo

AU - Graessmann, Monika

AU - Graessmann, Adolf

AU - Stocker, Ann Jacob

AU - Lara, F. J.S.

PY - 1993/12/20

Y1 - 1993/12/20

N2 - We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promoter region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.

AB - We have mapped the only transcription unit known to be present in the C-8 DNA puff of Rhynchosciara americana and describe the isolation and sequence of a cDNA clone, pRa C-8-22, which contains a nearly complete copy of the mRNA transcribed from this DNA puff and part of the sequence of genomic clone BSC8-0.9, which contains the promoter region and the remainder of the transcription unit. The characteristics of the protein predicted from the ORF present in the cDNA indicate that it is unique and secreted.

KW - C-8 cDNA

KW - C-8 mrNA

KW - DNA puff

KW - Rhynchosciara

KW - Salivary glands

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Molecular characterization of the DNA puff C-8 gene of Rhynchosciara americana

Abstract

Keywords

ASJC Scopus subject areas

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