Molecular characterization of human respiratory syncytial virus in the Philippines, 2012-2013

Rungnapa Malasao, Michiko Okamoto, Natthawan Chaimongkol, Tadatsugu Imamura, Kentaro Tohma, Isolde Dapat, Clyde Dapat, Akira Suzuki, Mayuko Saito, Mariko Saito, Raita Tamaki, Gay Anne Granada Pedrera-Rico, Rapunzel Aniceto, Reynaldo Frederick Negosa Quicho, Edelwisa Segubre-Mercado, Socorro Lupisan, Hitoshi Oshitani

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p

Original languageEnglish (US)
Article numbere0142192
JournalPLoS One
Issue number11
StatePublished - Nov 5 2015
Externally publishedYes

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General Biochemistry, Genetics and Molecular Biology
  • General Medicine


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