TY - JOUR
T1 - Molecular characterization of human respiratory syncytial virus in the Philippines, 2012-2013
AU - Malasao, Rungnapa
AU - Okamoto, Michiko
AU - Chaimongkol, Natthawan
AU - Imamura, Tadatsugu
AU - Tohma, Kentaro
AU - Dapat, Isolde
AU - Dapat, Clyde
AU - Suzuki, Akira
AU - Saito, Mayuko
AU - Saito, Mariko
AU - Tamaki, Raita
AU - Pedrera-Rico, Gay Anne Granada
AU - Aniceto, Rapunzel
AU - Quicho, Reynaldo Frederick Negosa
AU - Segubre-Mercado, Edelwisa
AU - Lupisan, Socorro
AU - Oshitani, Hitoshi
PY - 2015/11/5
Y1 - 2015/11/5
N2 - Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p
AB - Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p
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U2 - 10.1371/journal.pone.0142192
DO - 10.1371/journal.pone.0142192
M3 - Article
AN - SCOPUS:84951864362
SN - 1932-6203
VL - 10
JO - PLoS One
JF - PLoS One
IS - 11
M1 - e0142192
ER -