TY - JOUR
T1 - Molecular analysis of a pro‐T cell clone transformed by Abelson‐murine leukemia virus, displaying progressive γδ T cell receptor gene rearrangement and surface expression
AU - Heuze, Françoise
AU - Pardoll, Drew
AU - Diez, Elisabeth
AU - Ezine, Sophie
AU - Jotereau, Francine
PY - 1992/8
Y1 - 1992/8
N2 - We present a molecular analysis of T cell differentiation in a set of clones derived from in vitro Abelson murine leukemia virus (A‐MuLV) infection of fetal liver cells. The parental clone had partial rearrangement of the β and γ loci and spontaneously displayed progressive rearrangement of Vγ genes during in vitro culture. Further differentiation of these clones leading to δ gene rearrangement and CD4 expression, then CD8, CD3 and T cell receptor γδ chain surface expression was obtained after intrathymic transfer followed by in vitro co‐culture with thymic tissue. These A‐MuLV clones, therefore, appear to represent a powerful model sytem for studying the early molecular events of T cell development at the clonal level.
AB - We present a molecular analysis of T cell differentiation in a set of clones derived from in vitro Abelson murine leukemia virus (A‐MuLV) infection of fetal liver cells. The parental clone had partial rearrangement of the β and γ loci and spontaneously displayed progressive rearrangement of Vγ genes during in vitro culture. Further differentiation of these clones leading to δ gene rearrangement and CD4 expression, then CD8, CD3 and T cell receptor γδ chain surface expression was obtained after intrathymic transfer followed by in vitro co‐culture with thymic tissue. These A‐MuLV clones, therefore, appear to represent a powerful model sytem for studying the early molecular events of T cell development at the clonal level.
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U2 - 10.1002/eji.1830220818
DO - 10.1002/eji.1830220818
M3 - Article
C2 - 1322302
AN - SCOPUS:0026750332
SN - 0014-2980
VL - 22
SP - 2077
EP - 2084
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 8
ER -