Modulation of J774.1 Macrophage L-arginine metabolism by intracellular Mycobacterium bovis BCG

Marcy A. Peteroy-Kelly, Vishwanath Venketaraman, Meliza Talaue, Anjali Seth, Nancy D. Connell

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Using a Mycobacterium bovis BCG mutant (AS1) lacking a Bacillus subtilis L-arginine transporter homolog, we demonstrate here that the interaction between intracellular mycobacteria and the macrophage with respect to L-arginine transport and metabolism is quite complex. Intracellular AS1 stimulates macrophage L-arginine transport and accumulates 2.5-fold more 3H label derived from L-arginine than does the wild type. These studies suggest that the accumulation of 3H label reflects the acquisition of metabolites of L-arginine produced by the macrophage.

Original languageEnglish (US)
Pages (from-to)1011-1015
Number of pages5
JournalInfection and immunity
Volume71
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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