TY - JOUR
T1 - Mitochondria and apicoplast of Plasmodium falciparum
T2 - Behaviour on subcellular fractionation and the implication
AU - Kobayashi, Tamaki
AU - Sato, Shigeharu
AU - Takamiya, Shinzaburo
AU - Komaki-Yasuda, Kanako
AU - Yano, Kazuhiko
AU - Hirata, Ayami
AU - Onitsuka, Izumi
AU - Hata, Masayuki
AU - Mi-ichi, Fumika
AU - Tanaka, Takeshi
AU - Hase, Toshiharu
AU - Miyajima, Atsushi
AU - Kawazu, Shin ichiro
AU - Watanabe, Yoh ichi
AU - Kita, Kiyoshi
N1 - Funding Information:
The authors thank late Dr. Masamichi Aikawa for comments on morphological observations by electron microscopy and wish to dedicate this paper to him. The blood and plasma used in this study is a kind donation from Tokyo Metropolitan Red Cross Blood Centre. WR99210, the drug used for the screening of transfected parasites was a kind gift from Jacobus Pharmaceutical Co., Inc. This study was supported by a Grant-in-Aid for scientific research on priority areas and for Creative Scientific Research from the Japanese Ministry of Education, Science, Culture, and Sports (13226015, 13854011, 17209013, 17590368, 18073004 and 18GS0314) and for research on emerging and re-emerging infectious diseases from the Japanese Ministry of Health and Welfare, and by British Medical Research Council.
PY - 2007/2
Y1 - 2007/2
N2 - The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques - Percoll density gradient centrifugation and fluorescence-activated organelle sorting - for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium.
AB - The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques - Percoll density gradient centrifugation and fluorescence-activated organelle sorting - for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium.
KW - Apicoplast
KW - Fluorescence-activated organelle fractionation
KW - Mitochondrion
KW - Plasmodium falciparum
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U2 - 10.1016/j.mito.2006.11.021
DO - 10.1016/j.mito.2006.11.021
M3 - Article
C2 - 17289446
AN - SCOPUS:33847617920
SN - 1567-7249
VL - 7
SP - 125
EP - 132
JO - Mitochondrion
JF - Mitochondrion
IS - 1-2
ER -