@article{6160b6c149a8440ba149433ce3317dc3,
title = "miRNA-192 and -215 activate Wnt/β-catenin signaling pathway in gastric cancer via APC",
abstract = "Although great progress has been made in surgical techniques, traditional radiotherapy, and chemotherapy, gastric cancer (GC) is still the most common malignant tumor and has a high mortality, which highlights the importance of novel diagnostic markers. Emerging studies suggest that different microRNAs (miRNAs) are involved in tumorigenesis of GC. In this study, we found that miRNA-192 and -215 are significantly upregulated in GC and promote cell proliferation and migration. Adenomatous polyposis coli (APC), a well-known negative regulator in Wnt signaling, has been proved to be a target of miRNA-192 and -215. Inhibition of miRNA-192 or -215 reduced the Topflash activities and repressed the expression of Wnt signaling pathway proteins, while APC small interfering RNAs reversed the inhibitory effects, suggesting that miRNA-192 and -215 activate Wnt signaling via APC. In addition, APC mediates the cell proliferation and migration regulated by miRNA-192 and -215. Furthermore, APC is downregulated in GC tissues and negatively correlated with the expression of miRNA-192 and -215. In summary, miRNA-192 and -215 target APC and function as oncogenic miRNAs by activating Wnt signaling in GC, revealing to be potential therapeutic targets.",
keywords = "APC, Wnt signaling pathway, gastric cancer, miRNA-192 and -215",
author = "Shiqi Deng and Xiaojing Zhang and Ying Qin and Wangchun Chen and Hu Fan and Xianling Feng and Jian Wang and Ruibin Yan and Yanqiu Zhao and Yulan Cheng and Yanjie Wei and Xinmin Fan and Hassan Ashktorab and Duane Smoot and Meltzer, {Stephen J.} and Song Li and Kuan Li and Yin Peng and Zhe Jin",
note = "Funding Information: We are grateful to Drs. Hassan Ashktorab and Duane Smoot for the provision of HFE-145 cells. The present study was supported by the National Nature Science Foundation of China (grant no. 81772592) and the Science Technology and Innovation Committee of Shenzhen (grant no. JCYJ20170818142852491) to Z. Jin; the Guangdong Provincial Key Laboratory of Regional Immunity and Diseases (grant no. 2019B030301009); the National Natural Youth Science Foundation of China (grant no. 31601028), the Nature Science Foundation of Guangdong Province (grant no. 2017A030313144) and Startup Fund of Shenzhen University (grant no. 2018015) to Y. Peng; the Medical Science and Technology Research Foundation of Guangdong Province (grant no. A2016112), Nature Science Foundation of Guangdong Province (grant no. 2017A030313479) and The Planned Science and Technology Project of Shenzhen (grant no. JCYJ20160422170722474) to X. Zhang; the Guangdong Provincial Department of Science and Technology (grant no. 2016B090918122), the Science Technology and Innovation Committee of Shenzhen (grant nos. JCYJ20160331190123578 and GJHZ20170314154722613) to Y. Wei; and NIH grants DK087454, CA146799, and CA173390 and an American Cancer Society Clinical Research Professorship to Stephen J. Meltzer. Dr Meltzer is the Harry B. Myerberg-Thomas R. Hendrix Professor of Gastroenterology. Publisher Copyright: {\textcopyright} 2020 Wiley Periodicals, Inc.",
year = "2020",
month = sep,
day = "1",
doi = "10.1002/jcp.29550",
language = "English (US)",
volume = "235",
pages = "6218--6229",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "9",
}