miR-210 Expression Is Strongly Hypoxia-Induced in Anaplastic Thyroid Cancer Cell Lines and Is Associated with Extracellular Vesicles and Argonaute-2

Bonita H. Powell, Andrey Turchinovich, Yongchun Wang, Olesia Gololobova, Dominik Buschmann, Martha A. Zeiger, Christopher B. Umbricht, Kenneth W. Witwer

Research output: Contribution to journalArticlepeer-review

Abstract

Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and is believed to promote resistance to chemotherapy and radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach to treating aggressive cancers. Here, we explore the potential of the well-known hypoxia-responsive microRNA (miRNA) miR-210-3p as a cellular and extracellular biological marker of hypoxia. We compare miRNA expression across several ATC and papillary thyroid cancer (PTC) cell lines. In the ATC cell line SW1736, miR-210-3p expression levels indicate hypoxia during exposure to low oxygen conditions (2% O2). Furthermore, when released by SW1736 cells into the extracellular space, miR-210-3p is associated with RNA carriers such as extracellular vesicles (EVs) and Argonaute-2 (AGO2), making it a potential extracellular marker for hypoxia.

Original languageEnglish (US)
Article number4507
JournalInternational journal of molecular sciences
Volume24
Issue number5
DOIs
StatePublished - Mar 2023

Keywords

  • HIF1-alpha
  • anaplastic thyroid cancer
  • extracellular vesicles
  • hypoxia
  • miR-210
  • miRNA
  • microRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Spectroscopy
  • Catalysis
  • Inorganic Chemistry
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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