Minimally-invasive technique for injection into rat optic nerve

Kateryna Raykova; Melina V. Jones; Hwa Huang; Paul F. Hoffman; Michael Levy

doi:10.3791/52249

Minimally-invasive technique for injection into rat optic nerve

Kateryna Raykova, Melina V. Jones, Hwa Huang, Paul F. Hoffman, Michael Levy

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

Original language	English (US)
Article number	e52249
Journal	Journal of Visualized Experiments
Volume	2015
Issue number	99
DOIs	https://doi.org/10.3791/52249
State	Published - May 19 2015

Keywords

Injection
Issue 99
Neuroscience
Optic nerve
Optic neuritis
Optic neuropathy
Regeneration
Stem cells

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.3791/52249

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title = "Minimally-invasive technique for injection into rat optic nerve",

abstract = "The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.",

keywords = "Injection, Issue 99, Neuroscience, Optic nerve, Optic neuritis, Optic neuropathy, Regeneration, Stem cells",

author = "Kateryna Raykova and Jones, {Melina V.} and Hwa Huang and Hoffman, {Paul F.} and Michael Levy",

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AU - Huang, Hwa

AU - Hoffman, Paul F.

AU - Levy, Michael

PY - 2015/5/19

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AB - The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

KW - Injection

KW - Issue 99

KW - Neuroscience

KW - Optic nerve

KW - Optic neuritis

KW - Optic neuropathy

KW - Regeneration

KW - Stem cells

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Minimally-invasive technique for injection into rat optic nerve

Abstract

Keywords

ASJC Scopus subject areas

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