Minimally-invasive technique for injection into rat optic nerve

Kateryna Raykova, Melina V. Jones, Hwa Huang, Paul F. Hoffman, Michael Levy

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.

Original languageEnglish (US)
Article numbere52249
JournalJournal of Visualized Experiments
Issue number99
StatePublished - May 19 2015


  • Injection
  • Issue 99
  • Neuroscience
  • Optic nerve
  • Optic neuritis
  • Optic neuropathy
  • Regeneration
  • Stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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