Abstract
The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central nervous system in a minimally-invasive surgery without bone removal. This technique describes an approach to visualization and direct injection of the optic nerve following minor fascial dissection from the orbital ridge, using a conjunctival traction suture to gently pull the eye down and out. Representative examples of an injected optic nerve show successful injection of dyed beads.
Original language | English (US) |
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Article number | e52249 |
Journal | Journal of Visualized Experiments |
Volume | 2015 |
Issue number | 99 |
DOIs | |
State | Published - May 19 2015 |
Keywords
- Injection
- Issue 99
- Neuroscience
- Optic nerve
- Optic neuritis
- Optic neuropathy
- Regeneration
- Stem cells
ASJC Scopus subject areas
- Neuroscience(all)
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)