Abstract
Midkine, but not pleiotrophin, is mitogenic to human Wilms' tumor cells (G401 line) in dose-dependent and time-dependent fashion. Midkine specifically binds to high affinity (Kd = 0.15 +/- 0.02 nM, 210 kDa) and low affinity receptors (Kd = 0.65 +/- 0.07 nM, 75 kDa) on G401 cell surface, that has been confirmed by cross-linking and competition experiments. In addition, midkine stimulates a tyrosine phosphorylation of several proteins with molecular weight about 110-115 kDa, 130-140 kDa and 210 kDa. These data allow us to suggest that a key point in stimulation of G401 cell proliferation is interaction of midkine to its signaling receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 425-431 |
| Number of pages | 7 |
| Journal | Cellular and molecular biology (Noisy-le-Grand, France) |
| Volume | 43 |
| Issue number | 3 |
| State | Published - May 1997 |
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology
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