Microtubule Changes and Cytotoxicity in Leukemic Cell Lines Treated with Taxol

Eric K. Rowinsky, Ross C. Donehower, Richard J. Jones, Robert W. Tucker

Research output: Contribution to journalArticlepeer-review

364 Scopus citations


Taxol, a diterpenoid plant product that enhances the polymerization of tubulin, is currently entering clinical trials in the treatment of human leukemia. In order to develop an in vitro assay to predict tumor sensitivity to taxol, human leukemic cell lines were exposed to clinically achievable concentrations of taxol for relevant exposure periods. Changes in micro-tubules visualized by indirect immunofluorescence were compared to drug sensitivity measured by a clonogenic assay. Taxol produced either multiple mitotic asters in G2/M or microtubule bundling throughout the cell cycle. In cells that were relatively resistant to taxol, microtubule bundling was reversible while microtubule bundling in relatively sensitive cells persisted in the presence or absence of taxol. In contrast, aster formation was unrelated to cytotoxicity in any cell line. In the future, these microtubule effects may be useful in predicting the chemotherapeutic efficacy of taxol.

Original languageEnglish (US)
Pages (from-to)4093-4100
Number of pages8
JournalCancer Research
Issue number14
StatePublished - 1988

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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