Microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery

Xiangying Du, Yuesong Yang, Catherine Le Visage, Hunter H. Chen, Robert De Jong, Bensheng Qiu, Danming Wang, Kam W. Leong, Ulrike M. Hamper, Xiaoming Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The purpose of this study was to test the feasibility of using a novel biodegradable microsphere as a contrast agent/gene vector in ultrasound imaging-based vascular gene delivery. We first encapsulated green fluoresent protein (GFP) gene into the microspheres and validated the echogenic property of GFP-plasmid/microspheres with intramuscular injection. Then, the GFP-plasmid/microspheres were transferred into the arteries of pigs via a catheter-based delivery procedure under ultrasound imaging. Strong echogenic signal reflected from the microspheres indicated the destination of genes delivered, whereas functional gene expression was confirmed by immunohistochemical examination. Microspheres can be feasible contrast agents/gene vectors in ultrasound imaging-based vascular gene delivery in vivo.

Original languageEnglish (US)
Title of host publication2002 IEEE International Symposium on Biomedical Imaging, ISBI 2002 - Proceedings
PublisherIEEE Computer Society
Pages989-992
Number of pages4
ISBN (Electronic)078037584X
DOIs
StatePublished - 2002
EventIEEE International Symposium on Biomedical Imaging, ISBI 2002 - Washington, United States
Duration: Jul 7 2002Jul 10 2002

Publication series

NameProceedings - International Symposium on Biomedical Imaging
Volume2002-January
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Other

OtherIEEE International Symposium on Biomedical Imaging, ISBI 2002
Country/TerritoryUnited States
CityWashington
Period7/7/027/10/02

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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