Microscale pressure measurements based on an immiscible fluid/fluid interface

Jing Yang; Xing Duan; Andrew K. Fraser; Mohammad Ikbal Choudhury; Andrew J. Ewald; Rong Li; Sean X. Sun

doi:10.1038/s41598-019-56573-x

Microscale pressure measurements based on an immiscible fluid/fluid interface

Jing Yang, Xing Duan, Andrew K. Fraser, Mohammad Ikbal Choudhury, Andrew J. Ewald, Rong Li, Sean X. Sun

School of Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.

Original language	English (US)
Article number	20044
Journal	Scientific reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-56573-x
State	Published - Dec 1 2019

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title = "Microscale pressure measurements based on an immiscible fluid/fluid interface",

abstract = "A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.",

author = "Jing Yang and Xing Duan and Fraser, {Andrew K.} and Choudhury, {Mohammad Ikbal} and Ewald, {Andrew J.} and Rong Li and Sun, {Sean X.}",

note = "Funding Information: This research work was funded by the National Institutes of Health Grants R01GM114674 and U54CA210172 (S.X.S.). Partial support from the Fundamental Research Funds for the Central Universities in China under Grant No. LZUJBKY-2018-32 (J.Y.) is also gratefully acknowledged. Publisher Copyright: {\textcopyright} 2019, The Author(s).",

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doi = "10.1038/s41598-019-56573-x",

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AU - Yang, Jing

AU - Duan, Xing

AU - Fraser, Andrew K.

AU - Choudhury, Mohammad Ikbal

AU - Ewald, Andrew J.

AU - Li, Rong

AU - Sun, Sean X.

N1 - Funding Information: This research work was funded by the National Institutes of Health Grants R01GM114674 and U54CA210172 (S.X.S.). Partial support from the Fundamental Research Funds for the Central Universities in China under Grant No. LZUJBKY-2018-32 (J.Y.) is also gratefully acknowledged. Publisher Copyright: © 2019, The Author(s).

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N2 - A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.

AB - A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.

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Microscale pressure measurements based on an immiscible fluid/fluid interface

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