Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

Minghan Chen; Natalya V. Zaytseva; Qi Wu; Min Li; Ye Fang

doi:10.1063/1.4805041

Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

Minghan Chen, Natalya V. Zaytseva, Qi Wu, Min Li, Ye Fang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β₂-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

Original language	English (US)
Article number	193702
Journal	Applied Physics Letters
Volume	102
Issue number	19
DOIs	https://doi.org/10.1063/1.4805041
State	Published - May 13 2013
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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title = "Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology",

abstract = "We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.",

author = "Minghan Chen and Zaytseva, {Natalya V.} and Qi Wu and Min Li and Ye Fang",

note = "Funding Information: This work was supported partially by National Institutes of Health under Grant 5U54MH084691 (M.L., Y.F.).",

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doi = "10.1063/1.4805041",

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T1 - Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

AU - Chen, Minghan

AU - Zaytseva, Natalya V.

AU - Wu, Qi

AU - Li, Min

AU - Fang, Ye

N1 - Funding Information: This work was supported partially by National Institutes of Health under Grant 5U54MH084691 (M.L., Y.F.).

PY - 2013/5/13

Y1 - 2013/5/13

N2 - We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

AB - We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.

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Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

Abstract

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