TY - JOUR
T1 - Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting
AU - Miri, Amir K.
AU - Nieto, Daniel
AU - Iglesias, Luis
AU - Goodarzi Hosseinabadi, Hossein
AU - Maharjan, Sushila
AU - Ruiz-Esparza, Guillermo U.
AU - Khoshakhlagh, Parastoo
AU - Manbachi, Amir
AU - Dokmeci, Mehmet Remzi
AU - Chen, Shaochen
AU - Shin, Su Ryon
AU - Zhang, Yu Shrike
AU - Khademhosseini, Ali
N1 - Funding Information:
D.N., L.I., H.G.H., and S.M. contributed equally to this work. The authors gratefully acknowledge funding from the Office of Naval Research Young National Investigator Award, the National Institutes of Health (AR057837, DE021468, D005865, AR068258, AR066193, EB022403, EB021148, and EB021857), and the Presidential Early Career Award for Scientists and Engineers (PECASE). Y.S.Z. acknowledges the National Cancer Institute of the National Institutes of Health Pathway to Independence Award (K99CA201603). A.K.M. acknowledges the Fonds de recherche du Québec – Santé (FRQS) postdoctoral fellowship and Canadian Institutes of Health Research (CIHR). D.N. acknowledges (I2C plan) Xunta de Galicia funding. H.G.H. also thanks Prof. Reza Bagheri (Sharif University of Technology, Tehran, Iran) for his support. S.R.S. would like to recognize and thank Brigham and Women's Hospital President Betsy Nabel, MD, and the Reny family, for the Stepping Strong Innovator Award through their generous funding, and and Air Force Office of Sponsored Research under award # FA9550-15-1-0273. The authors acknowledge Dr. Farideh Davoudi (Brigham and Women's Hospital, Harvard Medical School) for her contributions in performing cell assays and Mr. Xichi Wang (Brigham and Women's Hospital, Harvard Medical School) for his contributions in the animal study. All animal experiments were conducted according to the NIH Guidelines for the Care and Use of Laboratory Animals. Protocol was approved by the Institutional Animal Care and Use Committee of Brigham and Women's Hospital (#2017N000114).
Funding Information:
D.N., L.I., H.G.H., and S.M. contributed equally to this work. The authors gratefully acknowledge funding from the Office of Naval Research Young National Investigator Award, the National Institutes of Health (AR057837, DE021468, D005865, AR068258, AR066193, EB022403, EB021148, and EB021857), and the Presidential Early Career Award for Scientists and Engineers (PECASE). Y.S.Z. acknowledges the National Cancer Institute of the National Institutes of Health Pathway to Independence Award (K99CA201603). A.K.M. acknowledges the Fonds de recherche du Québec – Santé (FRQS) postdoctoral fellowship and Canadian Institutes of Health Research (CIHR). D.N. acknowledges (I2C plan) Xunta de Galicia funding. H.G.H. also thanks Prof. Reza Bagheri (Sharif University of Technology, Tehran, Iran) for his support. S.R.S. would like to recognize and thank Brigham and Women’s Hospital President Betsy Nabel, MD, and the Reny family, for the Stepping Strong Innovator Award through their generous funding, and and Air Force Office of Sponsored Research under award # FA9550-15-1-0273. The authors acknowledge Dr. Farideh Davoudi (Brigham and Women’s Hospital, Harvard Medical School) for her contributions in performing cell assays and Mr. Xichi Wang (Brigham and Women’s Hospital, Harvard Medical School) for his contributions in the animal study. All animal experiments were conducted according to the NIH Guidelines for the Care and Use of Laboratory Animals. Protocol was approved by the Institutional Animal Care and Use Committee of Brigham and Women’s Hospital (#2017N000114).
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/7/5
Y1 - 2018/7/5
N2 - A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell-laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms.
AB - A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized by a moving stage and a microfluidic device containing four on/off pneumatic valves, is used to create 3D constructs. The novel microfluidic device is capable of fast switching between different (cell-loaded) hydrogel bioinks, to achieve layer-by-layer multimaterial bioprinting. Compared to conventional stereolithography-based bioprinters, the system provides the unique advantage of multimaterial fabrication capability at high spatial resolution. To demonstrate the multimaterial capacity of this system, a variety of hydrogel constructs are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) and gelatin methacryloyl (GelMA). The biocompatibility of this system is validated by introducing cell-laden GelMA into the microfluidic device and fabricating cellularized constructs. A pattern of a PEGDA frame and three different concentrations of GelMA, loaded with vascular endothelial growth factor, are further assessed for its neovascularization potential in a rat model. The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms.
KW - bioprinting
KW - digital light prototyping
KW - digital micromirror devices
KW - microfluidics
KW - multimaterials
UR - http://www.scopus.com/inward/record.url?scp=85046541321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046541321&partnerID=8YFLogxK
U2 - 10.1002/adma.201800242
DO - 10.1002/adma.201800242
M3 - Article
C2 - 29737048
AN - SCOPUS:85046541321
SN - 0935-9648
VL - 30
JO - Advanced Materials
JF - Advanced Materials
IS - 27
M1 - 1800242
ER -