Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata

Aling Dong; Min Ye; Hongzhu Guo; Junhua Zheng; Dean Guo

doi:10.1023/A:1022320824000

Microbial transformation of ginsenoside Rb₁ by Rhizopus stolonifer and Curvularia lunata

Aling Dong, Min Ye, Hongzhu Guo, Junhua Zheng, Dean Guo

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb₁, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (1), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ol (2), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (3), and 3-O-β-D-glucopyranosyl-3β, 12β, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, ¹H- and ¹³C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

Original language	English (US)
Pages (from-to)	339-344
Number of pages	6
Journal	Biotechnology Letters
Volume	25
Issue number	4
DOIs	https://doi.org/10.1023/A:1022320824000
State	Published - Feb 2003
Externally published	Yes

Keywords

Biotransformation
Curvularia lunata
Ginsenoside
Rhizopus stolonifer

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1023/A:1022320824000

Cite this

@article{db55f80a276d4ef3a162f3e9ece4e0d8,

title = "Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata",

abstract = "Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (1), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ol (2), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (3), and 3-O-β-D-glucopyranosyl-3β, 12β, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.",

keywords = "Biotransformation, Curvularia lunata, Ginsenoside, Rhizopus stolonifer",

author = "Aling Dong and Min Ye and Hongzhu Guo and Junhua Zheng and Dean Guo",

note = "Funding Information: We thank The National Outstanding Youth Foundation by NSF of China (39925040) and Trans-Century Training Program Foundation for the Talents by the Ministry of Education for financial support.",

year = "2003",

month = feb,

doi = "10.1023/A:1022320824000",

language = "English (US)",

volume = "25",

pages = "339--344",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "4",

}

TY - JOUR

T1 - Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata

AU - Dong, Aling

AU - Ye, Min

AU - Guo, Hongzhu

AU - Zheng, Junhua

AU - Guo, Dean

N1 - Funding Information: We thank The National Outstanding Youth Foundation by NSF of China (39925040) and Trans-Century Training Program Foundation for the Talents by the Ministry of Education for financial support.

PY - 2003/2

Y1 - 2003/2

N2 - Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (1), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ol (2), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (3), and 3-O-β-D-glucopyranosyl-3β, 12β, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

AB - Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (1), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-20-O- [β-D-glucopyranosyl]-3β,12β, 20(S)-trihydroxydammar-24-ol (2), 3-O-[β-D-glucopyranosyl-(1,2)-β-D-glucopyranosyl]-3β, 12β, 20(S)-trihydroxydammar-24-ene (3), and 3-O-β-D-glucopyranosyl-3β, 12β, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

KW - Biotransformation

KW - Curvularia lunata

KW - Ginsenoside

KW - Rhizopus stolonifer

UR - http://www.scopus.com/inward/record.url?scp=0037296157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037296157&partnerID=8YFLogxK

U2 - 10.1023/A:1022320824000

DO - 10.1023/A:1022320824000

M3 - Article

C2 - 12882549

AN - SCOPUS:0037296157

SN - 0141-5492

VL - 25

SP - 339

EP - 344

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -