TY - JOUR
T1 - Methylation-specific PCR.
AU - Herman, J. G.
AU - Baylin, S. B.
PY - 2001/5
Y1 - 2001/5
N2 - Methylation-specific PCR is a rapid method used to determine the methylation status of DNA. Not only does methylation affect the expression of genes in normal cells, but it is now known that disease processes such as cancer can result in abnormal DNA methylation patterns. Methylation-specific PCR can be used to investigate imprinted genes, to assess human tumors for clonality by studying genes inactivated on the X chromosome, and to examine abnormally methylated CpG islands in neoplasia.
AB - Methylation-specific PCR is a rapid method used to determine the methylation status of DNA. Not only does methylation affect the expression of genes in normal cells, but it is now known that disease processes such as cancer can result in abnormal DNA methylation patterns. Methylation-specific PCR can be used to investigate imprinted genes, to assess human tumors for clonality by studying genes inactivated on the X chromosome, and to examine abnormally methylated CpG islands in neoplasia.
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U2 - 10.1002/0471142905.hg1006s16
DO - 10.1002/0471142905.hg1006s16
M3 - Article
C2 - 18428243
AN - SCOPUS:44049105041
SN - 1934-8266
VL - Chapter 10
SP - Unit 10.6
JO - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
JF - Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
ER -