Methods to Monitor Cell Surface Expression and Endocytic Trafficking of CFTR in Polarized Epithelial Cells

Jennifer M. Bomberger, William B. Guggino, Bruce A. Stanton

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cystic fibrosis transmembrane conductance regulator (CFTR)-mediated chloride secretion is critical to maintaining airway surface hydration and efficient mucociliary clearance in the upper airways. Mutations in CFTR in cystic fibrosis lead to reduced expression of functional CFTR channels at the apical plasma membrane of the airway epithelium, leading to dehydration of the airway surface liquid and diminished mucociliary clearance. Cell surface CFTR is modulated by changes in CFTR endocytosis and recycling, effectively altering the cell surface abundance of the channel. This chapter examines current methods employed to measure the cell surface expression of CFTR, as well as methods to monitor CFTR movement through the endocytic pathway.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages271-283
Number of pages13
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume741
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cystic fibrosis
  • cystic fibrosis transmembrane conductance regulator
  • endocytosis
  • intracellular trafficking
  • recycling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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