Methods for swath™: Data independent acquisition on tripletof mass spectrometers

Ronald J. Holewinski; Sarah J. Parker; Andrea D. Matlock; Vidya Venkatraman; Jennifer E. Van Eyk

doi:10.1007/978-1-4939-3524-6_16

Methods for swath™: Data independent acquisition on tripletof mass spectrometers

Ronald J. Holewinski, Sarah J. Parker, Andrea D. Matlock, Vidya Venkatraman, Jennifer E. Van Eyk

School of Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex Triple TOF mass spectrometers and data analysis using the Sciex processing software.

Original language	English (US)
Pages (from-to)	265-279
Number of pages	15
Journal	Methods in Molecular Biology
Volume	1410
DOIs	https://doi.org/10.1007/978-1-4939-3524-6_16
State	Published - Feb 1 2016

Keywords

Data independent acquisition (DIA)
Mass spectrometry
Quantitative proteomics
Spectral ion library
Swath

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-3524-6_16

Cite this

@article{598bab08431442f3b9ac8ef2e3e527d0,

title = "Methods for swath{\texttrademark}: Data independent acquisition on tripletof mass spectrometers",

abstract = "Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex Triple TOF mass spectrometers and data analysis using the Sciex processing software.",

keywords = "Data independent acquisition (DIA), Mass spectrometry, Quantitative proteomics, Spectral ion library, Swath",

author = "Holewinski, {Ronald J.} and Parker, {Sarah J.} and Matlock, {Andrea D.} and Vidya Venkatraman and {Van Eyk}, {Jennifer E.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

month = feb,

day = "1",

doi = "10.1007/978-1-4939-3524-6_16",

language = "English (US)",

volume = "1410",

pages = "265--279",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Methods for swath™

T2 - Data independent acquisition on tripletof mass spectrometers

AU - Holewinski, Ronald J.

AU - Parker, Sarah J.

AU - Matlock, Andrea D.

AU - Venkatraman, Vidya

AU - Van Eyk, Jennifer E.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex Triple TOF mass spectrometers and data analysis using the Sciex processing software.

AB - Data independent acquisition (DIA also termed SWATH) is an emerging technology in the field of mass spectrometry based proteomics. Although the concept of DIA has been around for over a decade, the recent advancements, in particular the speed of acquisition, of mass analyzers have pushed the technique into the spotlight and allowed for high-quality DIA data to be routinely acquired by proteomics labs. In this chapter we will discuss the protocols used for DIA acquisition using the Sciex Triple TOF mass spectrometers and data analysis using the Sciex processing software.

KW - Data independent acquisition (DIA)

KW - Mass spectrometry

KW - Quantitative proteomics

KW - Spectral ion library

KW - Swath

UR - http://www.scopus.com/inward/record.url?scp=84958568727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958568727&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3524-6_16

DO - 10.1007/978-1-4939-3524-6_16

M3 - Article

C2 - 26867750

AN - SCOPUS:84958568727

SN - 1064-3745

VL - 1410

SP - 265

EP - 279

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Methods for swath™: Data independent acquisition on tripletof mass spectrometers

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this