Method validation for measurement of hair nicotine level in nonsmokers

Sung Roul Kim, Heather Wipfli, Erika Avila-Tang, Jonathan M. Samet, Patrick N. Breysse

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


The development of strategies to address the growing worldwide burden of exposure to secondhand smoke (SHS) would be facilitated by sensitive and accurate methods for assessing SHS exposure. Hair provides a readily available matrix for assessing biomarkers of typical SHS exposure. We developed and applied an optimized analytical method using an isotope dilution gas chromatography-mass spectrometry (GC/MS) for hair nicotine measurement. The utility of this optimized method is illustrated by presenting data on SHS exposure of women and children from 31 countries. Using this isotope dilution method with spiked samples (3.3 ng/mg), we found that the greatest hair nicotine extraction efficiency was obtained with a 60 min shaking time. In the field study (n = 2400), a positive association was evident between hair nicotine concentrations from nonsmokers and higher numbers of cigarettes smoked per day in a household.

Original languageEnglish (US)
Pages (from-to)273-279
Number of pages7
JournalBiomedical Chromatography
Issue number3
StatePublished - 2009


  • Children
  • Gas chromatography-mass spectrometry
  • Hair
  • Nicotine
  • Secondhand smoke

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry


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