Metabolomic Profiling of the Secretome from Human Neural Stem Cells Flown into Space

Juan Carlos Biancotti; Araceli Espinosa-Jeffrey

doi:10.3390/bioengineering11010011

Metabolomic Profiling of the Secretome from Human Neural Stem Cells Flown into Space

Juan Carlos Biancotti, Araceli Espinosa-Jeffrey

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The change in gravitational force has a significant effect on biological tissues and the entire organism. As with any alteration in the environment, microgravity (µG) produces modifications in the system inducing adaptation to the new condition. In this study, we analyzed the effect of µG on neural stem cells (NSCs) following a space flight to the International Space Station (ISS). After 3 days in space, analysis of the metabolome in culture medium revealed increased glycolysis with augmented pyruvate and glycerate levels, and activated catabolism of branched-chain amino acids (BCAA) and glutamine. NSCs flown into space (SPC-NSCs) also showed increased synthesis of NADH and formation of polyamine spermidine when compared to ground controls (GC-NSCs). Overall, the space environment appears to increase energy demands in response to the µG setting.

Original language	English (US)
Article number	11
Journal	Bioengineering
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/bioengineering11010011
State	Published - Jan 2024

Keywords

cell metabolism
metabolomics
microgravity
neural stem cells
secretome
spaceflight

ASJC Scopus subject areas

Bioengineering

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10.3390/bioengineering11010011

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abstract = "The change in gravitational force has a significant effect on biological tissues and the entire organism. As with any alteration in the environment, microgravity (µG) produces modifications in the system inducing adaptation to the new condition. In this study, we analyzed the effect of µG on neural stem cells (NSCs) following a space flight to the International Space Station (ISS). After 3 days in space, analysis of the metabolome in culture medium revealed increased glycolysis with augmented pyruvate and glycerate levels, and activated catabolism of branched-chain amino acids (BCAA) and glutamine. NSCs flown into space (SPC-NSCs) also showed increased synthesis of NADH and formation of polyamine spermidine when compared to ground controls (GC-NSCs). Overall, the space environment appears to increase energy demands in response to the µG setting.",

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Metabolomic Profiling of the Secretome from Human Neural Stem Cells Flown into Space

Abstract

Keywords

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