Abstract
Rat liver cells, isolated by chelate perfusion and extrusion through a tissue press, incorporated labeled acetate into cellular lipids and into lipids released into the suspending medium. Optimal rates of incorporation required supplementation of tris-KCl medium with Mg++, Mn++, succinate, citrate, nicotinamide, Coenzyme A, NADP and glucose-6-phosphate. The rate of acetate incorporation was markedly altered by changes in incubation media; tris-KCl was the most effective buffer. All the major classes of cellular lipids were labeled. ATP, BSA, inorganic phosphate, Ca++, 2,4-dinitrophenol and sodium clofibrate were potent inhibitors of acetate incorporation. When added to the incubation mixture, several hormones altered the rate of acetate incorporation into lipids.
Original language | English (US) |
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Pages (from-to) | 601-608 |
Number of pages | 8 |
Journal | Lipids |
Volume | 6 |
Issue number | 8 |
DOIs | |
State | Published - Aug 1971 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology