TY - JOUR
T1 - Metabolic glycoengineering bacteria for therapeutic, recombinant protein, and metabolite production applications
AU - Saeui, Christopher T.
AU - Urias, Esteban
AU - Liu, Lingshu
AU - Mathew, Mohit P.
AU - Yarema, Kevin J.
N1 - Funding Information:
Funding was obtained from the National Institutes of Health, NCI grant R01CA112314.
Publisher Copyright:
© 2015 Springer Science+Business Media New York.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Metabolic glycoengineering is a specialization of metabolic engineering that focuses on using small molecule metabolites to manipulate biosynthetic pathways responsible for oligosaccharide and glycoconjugate production. As outlined in this article, this technique has blossomed in mammalian systems over the past three decades but has made only modest progress in prokaryotes. Nevertheless, a sufficient foundation now exists to support several important applications of metabolic glycoengineering in bacteria based on methods to preferentially direct metabolic intermediates into pathways involved in lipopolysaccharide, peptidoglycan, teichoic acid, or capsule polysaccharide production. An overview of current applications and future prospects for this technology are provided in this report.
AB - Metabolic glycoengineering is a specialization of metabolic engineering that focuses on using small molecule metabolites to manipulate biosynthetic pathways responsible for oligosaccharide and glycoconjugate production. As outlined in this article, this technique has blossomed in mammalian systems over the past three decades but has made only modest progress in prokaryotes. Nevertheless, a sufficient foundation now exists to support several important applications of metabolic glycoengineering in bacteria based on methods to preferentially direct metabolic intermediates into pathways involved in lipopolysaccharide, peptidoglycan, teichoic acid, or capsule polysaccharide production. An overview of current applications and future prospects for this technology are provided in this report.
KW - Carbohydrate engineering
KW - Lipopolysaccharides
KW - Metabolic oligosaccharide engineering
KW - Nucleotide sugar production
KW - Oligosaccharide synthesis
KW - Petidoglycans
KW - Recombinant glycoprotein production
UR - http://www.scopus.com/inward/record.url?scp=84944279072&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84944279072&partnerID=8YFLogxK
U2 - 10.1007/s10719-015-9583-9
DO - 10.1007/s10719-015-9583-9
M3 - Article
C2 - 25931032
AN - SCOPUS:84944279072
SN - 0282-0080
VL - 32
SP - 425
EP - 441
JO - Glycoconjugate Journal
JF - Glycoconjugate Journal
IS - 7
ER -