TY - JOUR
T1 - Metabolic fluxes regulate the success of sporulation in Saccharomyces cerevisiae
AU - Aon, Juan C.
AU - Rapisarda, Viviana A.
AU - Cortassa, Sonia
N1 - Funding Information:
The authors thank Dr. M. A. Aon for many stimulating discussions and critical reading of the manuscript. This work was supported by grants from FundacioÂn Antorchas and Consejo de Investigaciones de la Universidad Nacional de TucumaÂn (CIUNT). S.C. is a career researcher from the Consejo Nacional de Investigaciones CientÂõ®cas y TeÂcnicas (CONICET) and J.C.A. is a fellow from CONICET.
PY - 1996/1/10
Y1 - 1996/1/10
N2 - In this work we investigated to what extent cellular metabolism and energetics regulate sporulation in Saccharomyces cerevisiae and which metabolic pathways are involved in such regulation. Sporulation, meiosis, and associated metabolic fluxes in S. cerevisiae strain CH1211 were studied in several experimental protocols involving changes of carbon source (acetate, lactate, or pyruvate) or cell density in sporulation medium, or changing the phase of batch growth at which cells were harvested before transfer to sporulation medium. In acetate-based sporulation medium, the rate at which cells utilized glyoxylate and gluconeogenic pathways correlated positively with the percentage of asci per cell at 72 h. In contrast, in lactate sporulation medium the frequency of sporulation correlated negatively with both the rate of lactate consumption and the fluxes through gluconeogenesis and the pyruvate-carboxylase catalyzed step. In the presence of lactate, the respiratory capacity did correlate positively with the percentage of asci per cell. The experimental data suggest that acetate limits fluxes to anabolic precursors during sporulation. In contrast, sporulation on lactate appears to be influenced by catabolic processes or, even more precisely, by the respiratory capacity of yeast cells. The results obtained are discussed in terms of the hypothesis that an imbalance between anabolic and catabolic fluxes may be required for an efficient sporulation.
AB - In this work we investigated to what extent cellular metabolism and energetics regulate sporulation in Saccharomyces cerevisiae and which metabolic pathways are involved in such regulation. Sporulation, meiosis, and associated metabolic fluxes in S. cerevisiae strain CH1211 were studied in several experimental protocols involving changes of carbon source (acetate, lactate, or pyruvate) or cell density in sporulation medium, or changing the phase of batch growth at which cells were harvested before transfer to sporulation medium. In acetate-based sporulation medium, the rate at which cells utilized glyoxylate and gluconeogenic pathways correlated positively with the percentage of asci per cell at 72 h. In contrast, in lactate sporulation medium the frequency of sporulation correlated negatively with both the rate of lactate consumption and the fluxes through gluconeogenesis and the pyruvate-carboxylase catalyzed step. In the presence of lactate, the respiratory capacity did correlate positively with the percentage of asci per cell. The experimental data suggest that acetate limits fluxes to anabolic precursors during sporulation. In contrast, sporulation on lactate appears to be influenced by catabolic processes or, even more precisely, by the respiratory capacity of yeast cells. The results obtained are discussed in terms of the hypothesis that an imbalance between anabolic and catabolic fluxes may be required for an efficient sporulation.
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U2 - 10.1006/excr.1996.0020
DO - 10.1006/excr.1996.0020
M3 - Article
C2 - 8549658
AN - SCOPUS:0030059970
SN - 0014-4827
VL - 222
SP - 157
EP - 162
JO - Experimental cell research
JF - Experimental cell research
IS - 1
ER -