TY - JOUR
T1 - Menadione-dependent alpha glycerophosphate and succinate dehydrogenases in the developing canine retina
AU - Mcleod, D. Scott
AU - Lutty, Gerard A.
N1 - Funding Information:
The authors wish to acknowledge Ms Lillian Fox for her expert editing and clerical skills, Dr Benjamin Goldberg for his guidance in histochemistry and Dr Arnall Patz for his continued support. This work was supported by NIH grants ## EY09357 (G.A.L.) and EY01765 (Wilmer). G. Lutty is an American Heart Association Established Investigator.
PY - 1995
Y1 - 1995
N2 - Reducing equivalents for the electron transport chain are generated within the mitochondria by the Krebs cycle and in cytoplasm by processes like lipid metabolism. Two mitochondrial enzymes, succinate dehydrogenase (SDH), a prominent enzyme in the Krebs cycle, and αglycerophosphate dehydrogenase (αGPDH), half of the glycerophosphate shuttle system for bringing reducing equivalents from cytoplasm to mitochondria, were examined enzyme histochemically to assess the contribution of each to metabolism of the developing canine retina. SDH activity, a common marker for oxidative metabolism, was insignificant at birth. By 4 days of age, activity was observed only in developing photoreceptor inner segments. By 21 days of age SDH activity was present throughout the retina, especially in photoreceptor inner segments and plexiform layers, and approached the level observed in the adult dog. Menadione-linked αGPDH (M-αGPDH) activity, however, was prominent in developing vasculature and outermost portion of the neuroblastic layer of the 1 day-old retina. Most notable was localization in vascular precursors, angioblasts, found distant from formed vessels in the peripheral nerve fiber layer. Retinal dependence on an oxidative metabolism in neuronal elements, as represented by SDH activity, occurs only when the vasculature is well established.
AB - Reducing equivalents for the electron transport chain are generated within the mitochondria by the Krebs cycle and in cytoplasm by processes like lipid metabolism. Two mitochondrial enzymes, succinate dehydrogenase (SDH), a prominent enzyme in the Krebs cycle, and αglycerophosphate dehydrogenase (αGPDH), half of the glycerophosphate shuttle system for bringing reducing equivalents from cytoplasm to mitochondria, were examined enzyme histochemically to assess the contribution of each to metabolism of the developing canine retina. SDH activity, a common marker for oxidative metabolism, was insignificant at birth. By 4 days of age, activity was observed only in developing photoreceptor inner segments. By 21 days of age SDH activity was present throughout the retina, especially in photoreceptor inner segments and plexiform layers, and approached the level observed in the adult dog. Menadione-linked αGPDH (M-αGPDH) activity, however, was prominent in developing vasculature and outermost portion of the neuroblastic layer of the 1 day-old retina. Most notable was localization in vascular precursors, angioblasts, found distant from formed vessels in the peripheral nerve fiber layer. Retinal dependence on an oxidative metabolism in neuronal elements, as represented by SDH activity, occurs only when the vasculature is well established.
KW - Alpha-glycerophosphate dehydrogenase
KW - Angioblasts
KW - Development
KW - Dog
KW - Menadione
KW - Retina
KW - Retinal vasculature
KW - Succinate dehydrogenase
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U2 - 10.3109/02713689508995804
DO - 10.3109/02713689508995804
M3 - Article
C2 - 8529421
AN - SCOPUS:0029099617
SN - 0271-3683
VL - 14
SP - 819
EP - 826
JO - Current Eye Research
JF - Current Eye Research
IS - 9
ER -