Menadione-dependent alpha glycerophosphate and succinate dehydrogenases in the developing canine retina

D. Scott Mcleod, Gerard A. Lutty

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Reducing equivalents for the electron transport chain are generated within the mitochondria by the Krebs cycle and in cytoplasm by processes like lipid metabolism. Two mitochondrial enzymes, succinate dehydrogenase (SDH), a prominent enzyme in the Krebs cycle, and αglycerophosphate dehydrogenase (αGPDH), half of the glycerophosphate shuttle system for bringing reducing equivalents from cytoplasm to mitochondria, were examined enzyme histochemically to assess the contribution of each to metabolism of the developing canine retina. SDH activity, a common marker for oxidative metabolism, was insignificant at birth. By 4 days of age, activity was observed only in developing photoreceptor inner segments. By 21 days of age SDH activity was present throughout the retina, especially in photoreceptor inner segments and plexiform layers, and approached the level observed in the adult dog. Menadione-linked αGPDH (M-αGPDH) activity, however, was prominent in developing vasculature and outermost portion of the neuroblastic layer of the 1 day-old retina. Most notable was localization in vascular precursors, angioblasts, found distant from formed vessels in the peripheral nerve fiber layer. Retinal dependence on an oxidative metabolism in neuronal elements, as represented by SDH activity, occurs only when the vasculature is well established.

Original languageEnglish (US)
Pages (from-to)819-826
Number of pages8
JournalCurrent Eye Research
Issue number9
StatePublished - 1995
Externally publishedYes


  • Alpha-glycerophosphate dehydrogenase
  • Angioblasts
  • Development
  • Dog
  • Menadione
  • Retina
  • Retinal vasculature
  • Succinate dehydrogenase

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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