Mechanisms that ensure speed and fidelity in eukaryotic translation termination

Michael R. Lawson, Laura N. Lessen, Jinfan Wang, Arjun Prabhakar, Nicholas C. Corsepius, Rachel Green, Joseph D. Puglisi

Research output: Contribution to journalArticlepeer-review


Translation termination, which liberates a nascent polypeptide from the ribosome specifically at stop codons, must occur accurately and rapidly. We established single-molecule fluorescence assays to track the dynamics of ribosomes and two requisite release factors (eRF1 and eRF3) throughout termination using an in vitro-reconstituted yeast translation system. We found that the two eukaryotic release factors bound together to recognize stop codons rapidly and elicit termination through a tightly regulated, multistep process that resembles transfer RNA selection during translation elongation. Because the release factors are conserved from yeast to humans, the molecular events that underlie yeast translation termination are likely broadly fundamental to eukaryotic protein synthesis.

Original languageEnglish (US)
Pages (from-to)876-882
Number of pages7
Issue number6557
StatePublished - Aug 20 2021

ASJC Scopus subject areas

  • General


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