Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions

Paul Steinberg; Philip S. Norman

doi:10.1016/0091-6749(73)90143-7

Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions

Paul Steinberg, Philip S. Norman

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

Original language	English (US)
Pages (from-to)	238-244
Number of pages	7
Journal	The Journal of allergy and clinical immunology
Volume	51
Issue number	4
DOIs	https://doi.org/10.1016/0091-6749(73)90143-7
State	Published - Apr 1973
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0091-6749(73)90143-7

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title = "Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions",

abstract = "An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.",

author = "Paul Steinberg and Norman, {Philip S.}",

note = "Funding Information: From the Division of Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine. Supported in part by a grant from the Allergy Foundation of America. Received for publication Oct. 3, 1972. Reprint requests to: Dr. Paul Steinberg, The University of{\textquoteleft} Michigan Medical Centrr, Depart-ment of Internal Medicine, Section of Allergy, Ann Arbor, Mich. 48104.",

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AU - Steinberg, Paul

AU - Norman, Philip S.

N1 - Funding Information: From the Division of Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine. Supported in part by a grant from the Allergy Foundation of America. Received for publication Oct. 3, 1972. Reprint requests to: Dr. Paul Steinberg, The University of‘ Michigan Medical Centrr, Depart-ment of Internal Medicine, Section of Allergy, Ann Arbor, Mich. 48104.

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N2 - An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

AB - An experimental model has been developed in which the stability of water-in-oil emulsions in simple environments of known constitution could be studied. A decrease in emulsion stability was effected by decreasing the pH of the emulsion environment with the ionic strength constant, increasing the ionic strength of the environment with the pH constant, or increasing the temperature of the environment with the pH and ionic strength constant. The concentration of protein in the emulsion environment at a given pH was not found to alter the stability of the emulsions. Parallels between in vitro influences upon stability and those produced by an inflammatory response at an emulsion repository site are discussed.

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Mechanism of action of mineral oil adjuvants. I. Conditions influencing the in vitro stability of water-in-oil emulsions

Abstract

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