Abstract
NusG/Spt5 proteins are the only transcription factors utilized by all cellular organisms. In enterobacteria, NusG antagonizes the transcription termination activity of Rho, a hexameric helicase, during the synthesis of ribosomal and actively translated mRNAs. Paradoxically, NusG helps Rho act on untranslated transcripts, including non-canonical antisense RNAs and those arising from translational stress; how NusG fulfills these disparate functions is unknown. Here, we demonstrate that NusG activates Rho by assisting helicase isomerization from an open-ring, RNA-loading state to a closed-ring, catalytically active translocase. A crystal structure of closed-ring Rho in complex with NusG reveals the physical basis for this activation and further explains how Rho is excluded from translationally competent RNAs. This study demonstrates how a universally conserved transcription factor acts to modulate the activity of a ring-shaped ATPase motor and establishes how the innate sequence bias of a termination factor can be modulated to silence pervasive, aberrant transcription. Lawson et al. show that NusG, a member of a universally conserved transcription factor family, helps isomerize the Rho transcription termination factor from an open-ring loading state to an active, closed-ring conformation. This action overrides the innate sequence bias of Rho to terminate aberrant transcriptional events emanating from translational stress.
Original language | English (US) |
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Pages (from-to) | 911-922.e4 |
Journal | Molecular cell |
Volume | 71 |
Issue number | 6 |
DOIs | |
State | Published - Sep 20 2018 |
Keywords
- ATPase
- NusG
- Rho
- Spt5
- termination
- transcription
- translation
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology