Measuring In Vivo Mitophagy

Nuo Sun, Jeanho Yun, Jie Liu, Daniela Malide, Chengyu Liu, Ilsa I. Rovira, Kira M. Holmström, Maria M. Fergusson, Young Hyun Yoo, Christian A. Combs, Toren Finkel

Research output: Contribution to journalArticlepeer-review

282 Scopus citations


Alterations in mitophagy have been increasingly linked to aging and age-related diseases. There are, however, no convenient methods to analyze mitophagy in vivo. Here, we describe a transgenic mouse model in which we expressed a mitochondrial-targeted form of the fluorescent reporter Keima (mt-Keima). Keima is a coral-derived protein that exhibits both pH-dependent excitation and resistance to lysosomal proteases. Comparison of a wide range of primary cells and tissues generated from the mt-Keima mouse revealed significant variations in basal mitophagy. In addition, we have employed the mt-Keima mice to analyze how mitophagy is altered by conditions including diet, oxygen availability, Huntingtin transgene expression, the absence of macroautophagy (ATG5 or ATG7 expression), an increase in mitochondrial mutational load, the presence of metastatic tumors, and normal aging. The ability to assess mitophagy under a host of varying environmental and genetic perturbations suggests that the mt-Keima mouse should be a valuable resource.

Original languageEnglish (US)
Pages (from-to)685-696
Number of pages12
JournalMolecular Cell
Issue number4
StatePublished - Nov 19 2015
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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