Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry

Breanna M. Tracey; Lakyn N. Mayo; Christopher T. Le; Victoria Y. Chen; Julian Weichsel; Corinne Y. Renner; Jiaqi Li; Joby J. Tsai; Sachin P. Kalarn; Michael T. Ou; Luke Y. Chang; Taariq K. Mohammed; Isa S.K. Mohammed; Khelly Shah; Ginger M. Thompson; Anja M.K. Jones; Lily T. Im; Mona A. Kaleem; Osamah J. Saeedi

doi:10.1038/s41598-019-56239-8

Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry

Breanna M. Tracey, Lakyn N. Mayo, Christopher T. Le, Victoria Y. Chen, Julian Weichsel, Corinne Y. Renner, Jiaqi Li, Joby J. Tsai, Sachin P. Kalarn, Michael T. Ou, Luke Y. Chang, Taariq K. Mohammed, Isa S.K. Mohammed, Khelly Shah, Ginger M. Thompson, Anja M.K. Jones, Lily T. Im, Mona A. Kaleem, Osamah J. Saeedi

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2 Scopus citations

Abstract

Changes in retinal blood flow may be involved in the pathogenesis of glaucoma and other ocular diseases. Erythrocyte mediated velocimetry (EMV) is a novel technique where indocyanine green (ICG) dye is sequestered in erythrocyte ghosts and autologously re-injected to allow direct visualization of erythrocytes for in vivo measurement of speed. The purpose of this study is to determine the mean erythrocyte speed in the retinal microvasculature, as well as the intravisit and intervisit variability of EMV. Data from 23 EMV sessions from control, glaucoma suspect, and glaucoma patients were included in this study. In arteries with an average diameter of 43.11 µm ± 6.62 µm, the mean speed was 7.17 mm/s ± 2.35 mm/s. In veins with an average diameter of 45.87 µm ± 12.04 µm, the mean speed was 6.05 mm/s ± 1.96 mm/s. Intravisit variability, as measured by the mean coefficient of variation, was 3.57% (range 0.44–9.68%). Intervisit variability was 4.85% (range 0.15–8.43%). EMV may represent reliable method for determination of retinal blood speed, potentially allowing insights into the effects of pharmacologic agents or pathogenesis of ocular diseases.

Original language	English (US)
Article number	20178
Journal	Scientific reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-56239-8
State	Published - Dec 1 2019
Externally published	Yes

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Tracey, B. M., Mayo, L. N., Le, C. T., Chen, V. Y., Weichsel, J., Renner, C. Y., Li, J., Tsai, J. J., Kalarn, S. P., Ou, M. T., Chang, L. Y., Mohammed, T. K., Mohammed, I. S. K., Shah, K., Thompson, G. M., Jones, A. M. K., Im, L. T., Kaleem, M. A., & Saeedi, O. J. (2019). Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry. Scientific reports, 9(1), Article 20178. https://doi.org/10.1038/s41598-019-56239-8

Tracey, BM, Mayo, LN, Le, CT, Chen, VY, Weichsel, J, Renner, CY, Li, J, Tsai, JJ, Kalarn, SP, Ou, MT, Chang, LY, Mohammed, TK, Mohammed, ISK, Shah, K, Thompson, GM, Jones, AMK, Im, LT, Kaleem, MA & Saeedi, OJ 2019, 'Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry', Scientific reports, vol. 9, no. 1, 20178. https://doi.org/10.1038/s41598-019-56239-8

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title = "Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry",

abstract = "Changes in retinal blood flow may be involved in the pathogenesis of glaucoma and other ocular diseases. Erythrocyte mediated velocimetry (EMV) is a novel technique where indocyanine green (ICG) dye is sequestered in erythrocyte ghosts and autologously re-injected to allow direct visualization of erythrocytes for in vivo measurement of speed. The purpose of this study is to determine the mean erythrocyte speed in the retinal microvasculature, as well as the intravisit and intervisit variability of EMV. Data from 23 EMV sessions from control, glaucoma suspect, and glaucoma patients were included in this study. In arteries with an average diameter of 43.11 µm ± 6.62 µm, the mean speed was 7.17 mm/s ± 2.35 mm/s. In veins with an average diameter of 45.87 µm ± 12.04 µm, the mean speed was 6.05 mm/s ± 1.96 mm/s. Intravisit variability, as measured by the mean coefficient of variation, was 3.57% (range 0.44–9.68%). Intervisit variability was 4.85% (range 0.15–8.43%). EMV may represent reliable method for determination of retinal blood speed, potentially allowing insights into the effects of pharmacologic agents or pathogenesis of ocular diseases.",

author = "Tracey, {Breanna M.} and Mayo, {Lakyn N.} and Le, {Christopher T.} and Chen, {Victoria Y.} and Julian Weichsel and Renner, {Corinne Y.} and Jiaqi Li and Tsai, {Joby J.} and Kalarn, {Sachin P.} and Ou, {Michael T.} and Chang, {Luke Y.} and Mohammed, {Taariq K.} and Mohammed, {Isa S.K.} and Khelly Shah and Thompson, {Ginger M.} and Jones, {Anja M.K.} and Im, {Lily T.} and Kaleem, {Mona A.} and Saeedi, {Osamah J.}",

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doi = "10.1038/s41598-019-56239-8",

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AU - Tracey, Breanna M.

AU - Mayo, Lakyn N.

AU - Le, Christopher T.

AU - Chen, Victoria Y.

AU - Weichsel, Julian

AU - Renner, Corinne Y.

AU - Li, Jiaqi

AU - Tsai, Joby J.

AU - Kalarn, Sachin P.

AU - Ou, Michael T.

AU - Chang, Luke Y.

AU - Mohammed, Taariq K.

AU - Mohammed, Isa S.K.

AU - Shah, Khelly

AU - Thompson, Ginger M.

AU - Jones, Anja M.K.

AU - Im, Lily T.

AU - Kaleem, Mona A.

AU - Saeedi, Osamah J.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Changes in retinal blood flow may be involved in the pathogenesis of glaucoma and other ocular diseases. Erythrocyte mediated velocimetry (EMV) is a novel technique where indocyanine green (ICG) dye is sequestered in erythrocyte ghosts and autologously re-injected to allow direct visualization of erythrocytes for in vivo measurement of speed. The purpose of this study is to determine the mean erythrocyte speed in the retinal microvasculature, as well as the intravisit and intervisit variability of EMV. Data from 23 EMV sessions from control, glaucoma suspect, and glaucoma patients were included in this study. In arteries with an average diameter of 43.11 µm ± 6.62 µm, the mean speed was 7.17 mm/s ± 2.35 mm/s. In veins with an average diameter of 45.87 µm ± 12.04 µm, the mean speed was 6.05 mm/s ± 1.96 mm/s. Intravisit variability, as measured by the mean coefficient of variation, was 3.57% (range 0.44–9.68%). Intervisit variability was 4.85% (range 0.15–8.43%). EMV may represent reliable method for determination of retinal blood speed, potentially allowing insights into the effects of pharmacologic agents or pathogenesis of ocular diseases.

AB - Changes in retinal blood flow may be involved in the pathogenesis of glaucoma and other ocular diseases. Erythrocyte mediated velocimetry (EMV) is a novel technique where indocyanine green (ICG) dye is sequestered in erythrocyte ghosts and autologously re-injected to allow direct visualization of erythrocytes for in vivo measurement of speed. The purpose of this study is to determine the mean erythrocyte speed in the retinal microvasculature, as well as the intravisit and intervisit variability of EMV. Data from 23 EMV sessions from control, glaucoma suspect, and glaucoma patients were included in this study. In arteries with an average diameter of 43.11 µm ± 6.62 µm, the mean speed was 7.17 mm/s ± 2.35 mm/s. In veins with an average diameter of 45.87 µm ± 12.04 µm, the mean speed was 6.05 mm/s ± 1.96 mm/s. Intravisit variability, as measured by the mean coefficient of variation, was 3.57% (range 0.44–9.68%). Intervisit variability was 4.85% (range 0.15–8.43%). EMV may represent reliable method for determination of retinal blood speed, potentially allowing insights into the effects of pharmacologic agents or pathogenesis of ocular diseases.

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Measurement of Retinal Microvascular Blood Velocity Using Erythrocyte Mediated Velocimetry

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