Measurement of plasma dihydrotestosterone by competitive protein-binding analysis

Roland R. Tremblay; Inese Z. Beitins; Avinoam Kowarski; Claude J. Migeon

doi:10.1016/S0039-128X(70)80093-9

Measurement of plasma dihydrotestosterone by competitive protein-binding analysis

Roland R. Tremblay, Inese Z. Beitins, Avinoam Kowarski, Claude J. Migeon

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A competitive protein binding method for the measurement of dihydrotestosterone (DHT) concentration in plasma is described. The mean recovery throughout the procedure was 75 ± 11%. Water blanks (10 ml) ranged from 0 to 0.2 ng per sample. The precision and accuracy were good. The specificity was checked by preparation of two further derivatives of DHT. Mean plasma values of DHT for normal adult women and men were respectively 21.7 ± 4.3 (S.D.) and 50.1 ± 14.4 (S.D.) ng/100 ml. Simultaneously by taking a smaller aliquot following the paper chromatography, plasma testosterone (T) concentrations could be measured by competitive protein binding.

Original language	English (US)
Pages (from-to)	29-40
Number of pages	12
Journal	Steroids
Volume	16
Issue number	C
DOIs	https://doi.org/10.1016/S0039-128X(70)80093-9
State	Published - 1970
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(70)80093-9

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title = "Measurement of plasma dihydrotestosterone by competitive protein-binding analysis",

abstract = "A competitive protein binding method for the measurement of dihydrotestosterone (DHT) concentration in plasma is described. The mean recovery throughout the procedure was 75 ± 11%. Water blanks (10 ml) ranged from 0 to 0.2 ng per sample. The precision and accuracy were good. The specificity was checked by preparation of two further derivatives of DHT. Mean plasma values of DHT for normal adult women and men were respectively 21.7 ± 4.3 (S.D.) and 50.1 ± 14.4 (S.D.) ng/100 ml. Simultaneously by taking a smaller aliquot following the paper chromatography, plasma testosterone (T) concentrations could be measured by competitive protein binding.",

author = "Tremblay, {Roland R.} and Beitins, {Inese Z.} and Avinoam Kowarski and Migeon, {Claude J.}",

note = "Funding Information: This investigation was supported by Research Grant AM-00180-19, Traineeship Grant TO1-AM-5219 and Research Career Award 5 K06-AM-21,855 (CJM) from the United States Public Health Service. Dr. Tremblay was supported by a Fellowship from the Medical Research Council of Canada.",

year = "1970",

doi = "10.1016/S0039-128X(70)80093-9",

language = "English (US)",

volume = "16",

pages = "29--40",

journal = "Steroids",

issn = "0039-128X",

publisher = "Elsevier Inc.",

number = "C",

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TY - JOUR

T1 - Measurement of plasma dihydrotestosterone by competitive protein-binding analysis

AU - Tremblay, Roland R.

AU - Beitins, Inese Z.

AU - Kowarski, Avinoam

AU - Migeon, Claude J.

N1 - Funding Information: This investigation was supported by Research Grant AM-00180-19, Traineeship Grant TO1-AM-5219 and Research Career Award 5 K06-AM-21,855 (CJM) from the United States Public Health Service. Dr. Tremblay was supported by a Fellowship from the Medical Research Council of Canada.

PY - 1970

Y1 - 1970

N2 - A competitive protein binding method for the measurement of dihydrotestosterone (DHT) concentration in plasma is described. The mean recovery throughout the procedure was 75 ± 11%. Water blanks (10 ml) ranged from 0 to 0.2 ng per sample. The precision and accuracy were good. The specificity was checked by preparation of two further derivatives of DHT. Mean plasma values of DHT for normal adult women and men were respectively 21.7 ± 4.3 (S.D.) and 50.1 ± 14.4 (S.D.) ng/100 ml. Simultaneously by taking a smaller aliquot following the paper chromatography, plasma testosterone (T) concentrations could be measured by competitive protein binding.

AB - A competitive protein binding method for the measurement of dihydrotestosterone (DHT) concentration in plasma is described. The mean recovery throughout the procedure was 75 ± 11%. Water blanks (10 ml) ranged from 0 to 0.2 ng per sample. The precision and accuracy were good. The specificity was checked by preparation of two further derivatives of DHT. Mean plasma values of DHT for normal adult women and men were respectively 21.7 ± 4.3 (S.D.) and 50.1 ± 14.4 (S.D.) ng/100 ml. Simultaneously by taking a smaller aliquot following the paper chromatography, plasma testosterone (T) concentrations could be measured by competitive protein binding.

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SP - 29

EP - 40

JO - Steroids

JF - Steroids

IS - C

ER -

Measurement of plasma dihydrotestosterone by competitive protein-binding analysis

Abstract

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