Abstract
A solid-phase extraction procedure was developed for the isolation of heroin, 6-acetylmorphine, and morphine from blood, plasma, saliva, and urine with subsequent assay by gas chromatography/mass spectrometry. Aprotic solvents, mild elution conditions, and an enzyme inhibitor were used to ensure maximum analyte stability. Samples were extracted and the extract was divided into two equal portions. One portion was assayed directly for heroin; detector response was linear over a concentration range of 1.0 to 250 μg/L. The second part of the extract was reacted with N-methyl-bis- trifluoroacetamide and assayed for the trifluoroacetyl derivatives of 6- acetylmorphine and morphine; detector response was linear over a concentration range of 1.0 to 500 μg/L. The limit of sensitivity was 1.0 μg/L for each analyte. Hydrolysis of heroin to 6-acetylmorphine during extraction and analysis was <5%. The method can be used to corroborate heroin use and to study the pharmacological effects of heroin and its metabolites.
Original language | English (US) |
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Pages (from-to) | 670-675 |
Number of pages | 6 |
Journal | Clinical chemistry |
Volume | 39 |
Issue number | 4 |
DOIs | |
State | Published - 1993 |
Externally published | Yes |
Keywords
- 6-acetylmorphine
- abused drugs
- gas chromatography/mass spectrometry
- metabolism
- morphine
- saliva
- solid-phase extraction
- urine
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical