MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS; G. N. HENDY; S. E. PAPAPOULOS; L. J. FRAHER; A. D. CARE; J. L.H. O'RIORDAN

doi:10.1111/j.1365-2265.1979.tb03068.x

MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY

T. L. CLEMENS, G. N. HENDY, S. E. PAPAPOULOS, L. J. FRAHER, A. D. CARE, J. L.H. O'RIORDAN

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Abstract

1α,25‐dihydroxycholecalciferol (1,25‐(OH)₂D₃) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH)₂D₃‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D₃ cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH)₂D₃ concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.

Original language	English (US)
Pages (from-to)	225-234
Number of pages	10
Journal	Clinical Endocrinology
Volume	11
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2265.1979.tb03068.x
State	Published - Aug 1979
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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title = "MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY",

abstract = "1α,25‐dihydroxycholecalciferol (1,25‐(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH)2D3‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH)2D3 concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.",

author = "CLEMENS, {T. L.} and HENDY, {G. N.} and PAPAPOULOS, {S. E.} and FRAHER, {L. J.} and CARE, {A. D.} and O'RIORDAN, {J. L.H.}",

year = "1979",

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doi = "10.1111/j.1365-2265.1979.tb03068.x",

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AU - CLEMENS, T. L.

AU - HENDY, G. N.

AU - PAPAPOULOS, S. E.

AU - FRAHER, L. J.

AU - CARE, A. D.

AU - O'RIORDAN, J. L.H.

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N2 - 1α,25‐dihydroxycholecalciferol (1,25‐(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH)2D3‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH)2D3 concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.

AB - 1α,25‐dihydroxycholecalciferol (1,25‐(OH)2D3) has been measured in human serum by radioimmunoassay. The assay uses a high titre antiserum raised in sheep against 1,25‐(OH)2D3‐25‐hemisuccinate, conjugated to bovine serum albumin. The sensitivity of the assay is 10 pg/tube. Other hydroxylated forms of vitamin D3 cross react with the antiserum and are therefore removed from serum extracts by chromatography on Sephadex LH 20 followed by high pressure liquid chromatography. The mean (± SEM) serum 1,25‐(OH)2D3 concentration for a group of healthy adult subjects was 41 ± 2.5 pg/ml. None was detected in anephric patients and the concentration was low or undetectable in patients with chronic renal failure.

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MEASUREMENT OF 1,25‐DIHYDROXYCHOLECALCIFEROL IN MAN BY RADIOIMMUNOASSAY

Abstract

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