Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays

Patricia M.E. Moore; Edward C. Hayes; Sara E. Miller; Larry L. Wright; Carolyn E. Machamer; Hans J. Zweerink

doi:10.1128/iai.20.3.842-846.1978

Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays

Patricia M.E. Moore, Edward C. Hayes, Sara E. Miller, Larry L. Wright, Carolyn E. Machamer, Hans J. Zweerink

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Nucleocapsids in quantities approaching 1 mg were purified from 10⁹ measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [³⁵S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

Original language	English (US)
Pages (from-to)	842-846
Number of pages	5
Journal	Unknown Journal
Volume	20
Issue number	3
DOIs	https://doi.org/10.1128/iai.20.3.842-846.1978
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/iai.20.3.842-846.1978

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title = "Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays",

abstract = "Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.",

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T2 - large-scale purification and use in radioimmunoassays

AU - Moore, Patricia M.E.

AU - Hayes, Edward C.

AU - Miller, Sara E.

AU - Wright, Larry L.

AU - Machamer, Carolyn E.

AU - Zweerink, Hans J.

PY - 1978

Y1 - 1978

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AB - Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbit against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead, partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.

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Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays

Abstract

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