Maturation of spontaneous firing properties after hearing onset in rat auditory nerve fibers: Spontaneous rates, refractoriness, and interfiber correlations

Jingjing Sherry Wu; Eric D. Young; Elisabeth Glowatzki

doi:10.1523/JNEUROSCI.1187-16.2016

Maturation of spontaneous firing properties after hearing onset in rat auditory nerve fibers: Spontaneous rates, refractoriness, and interfiber correlations

Jingjing Sherry Wu, Eric D. Young, Elisabeth Glowatzki

School of Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Auditory nerve fibers (ANFs) exhibit a range of spontaneous firing rates (SRs) that are inversely correlated with threshold for sounds. To probe the underlying mechanisms and time course ofSRdifferentiation during cochlear maturation, loose-patch extracellular recordings weremadefromANFdendrites using acutely excised rat cochlear preparations of different ages after hearing onset. Diversification of SRs occurred mostly between the second and the third postnatal week. Statistical properties of ANF spike trains showed developmental changes that approach adult-like features in older preparations. Comparison with intracellularly recorded EPSCs revealed that most properties of ANF spike trains derive from the characteristics of presynaptic transmitter release. Pharmacological tests and waveform analysis showed that endogenous firing produces some fraction of ANF spikes, accounting for their unusual properties; the endogenous firing diminishes gradually during maturation. Paired recordings showed that ANFs contacting the same inner hair cell could have different SRs, with no correlation in their spike timing.

Original language	English (US)
Pages (from-to)	10584-10597
Number of pages	14
Journal	Journal of Neuroscience
Volume	36
Issue number	41
DOIs	https://doi.org/10.1523/JNEUROSCI.1187-16.2016
State	Published - Oct 12 2016

Keywords

Auditory nerve fiber
Development
Hair cell
Intrinsic firing
Refractoriness
Spontaneous rate

ASJC Scopus subject areas

Neuroscience(all)

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10.1523/JNEUROSCI.1187-16.2016

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title = "Maturation of spontaneous firing properties after hearing onset in rat auditory nerve fibers: Spontaneous rates, refractoriness, and interfiber correlations",

abstract = "Auditory nerve fibers (ANFs) exhibit a range of spontaneous firing rates (SRs) that are inversely correlated with threshold for sounds. To probe the underlying mechanisms and time course ofSRdifferentiation during cochlear maturation, loose-patch extracellular recordings weremadefromANFdendrites using acutely excised rat cochlear preparations of different ages after hearing onset. Diversification of SRs occurred mostly between the second and the third postnatal week. Statistical properties of ANF spike trains showed developmental changes that approach adult-like features in older preparations. Comparison with intracellularly recorded EPSCs revealed that most properties of ANF spike trains derive from the characteristics of presynaptic transmitter release. Pharmacological tests and waveform analysis showed that endogenous firing produces some fraction of ANF spikes, accounting for their unusual properties; the endogenous firing diminishes gradually during maturation. Paired recordings showed that ANFs contacting the same inner hair cell could have different SRs, with no correlation in their spike timing.",

keywords = "Auditory nerve fiber, Development, Hair cell, Intrinsic firing, Refractoriness, Spontaneous rate",

author = "Wu, {Jingjing Sherry} and Young, {Eric D.} and Elisabeth Glowatzki",

note = "Funding Information: This work was supported by National Institute on Deafness and Other Communication Disorders Grants R01DC006476 and R01DC012957 to E.G. and National Institute on Deafness and Other Communication Disorders P30 DC005211 to the Center for Hearing and Balance at Johns Hopkins. We thank Tessa Ropp for cat ANF data in Figures 2 B and 4 E; Eunyoung Yi and Lisa Grant for recordings that were included in the analysis of EPSCs and AP waveforms; and Lisa Grant for initiating the project on simultaneous recordings of two fibers at one hair cell in the laboratory. The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2016 the authors.",

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doi = "10.1523/JNEUROSCI.1187-16.2016",

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AU - Young, Eric D.

AU - Glowatzki, Elisabeth

N1 - Funding Information: This work was supported by National Institute on Deafness and Other Communication Disorders Grants R01DC006476 and R01DC012957 to E.G. and National Institute on Deafness and Other Communication Disorders P30 DC005211 to the Center for Hearing and Balance at Johns Hopkins. We thank Tessa Ropp for cat ANF data in Figures 2 B and 4 E; Eunyoung Yi and Lisa Grant for recordings that were included in the analysis of EPSCs and AP waveforms; and Lisa Grant for initiating the project on simultaneous recordings of two fibers at one hair cell in the laboratory. The authors declare no competing financial interests. Publisher Copyright: © 2016 the authors.

PY - 2016/10/12

Y1 - 2016/10/12

N2 - Auditory nerve fibers (ANFs) exhibit a range of spontaneous firing rates (SRs) that are inversely correlated with threshold for sounds. To probe the underlying mechanisms and time course ofSRdifferentiation during cochlear maturation, loose-patch extracellular recordings weremadefromANFdendrites using acutely excised rat cochlear preparations of different ages after hearing onset. Diversification of SRs occurred mostly between the second and the third postnatal week. Statistical properties of ANF spike trains showed developmental changes that approach adult-like features in older preparations. Comparison with intracellularly recorded EPSCs revealed that most properties of ANF spike trains derive from the characteristics of presynaptic transmitter release. Pharmacological tests and waveform analysis showed that endogenous firing produces some fraction of ANF spikes, accounting for their unusual properties; the endogenous firing diminishes gradually during maturation. Paired recordings showed that ANFs contacting the same inner hair cell could have different SRs, with no correlation in their spike timing.

AB - Auditory nerve fibers (ANFs) exhibit a range of spontaneous firing rates (SRs) that are inversely correlated with threshold for sounds. To probe the underlying mechanisms and time course ofSRdifferentiation during cochlear maturation, loose-patch extracellular recordings weremadefromANFdendrites using acutely excised rat cochlear preparations of different ages after hearing onset. Diversification of SRs occurred mostly between the second and the third postnatal week. Statistical properties of ANF spike trains showed developmental changes that approach adult-like features in older preparations. Comparison with intracellularly recorded EPSCs revealed that most properties of ANF spike trains derive from the characteristics of presynaptic transmitter release. Pharmacological tests and waveform analysis showed that endogenous firing produces some fraction of ANF spikes, accounting for their unusual properties; the endogenous firing diminishes gradually during maturation. Paired recordings showed that ANFs contacting the same inner hair cell could have different SRs, with no correlation in their spike timing.

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Maturation of spontaneous firing properties after hearing onset in rat auditory nerve fibers: Spontaneous rates, refractoriness, and interfiber correlations

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